一种新型冻干骨材料的安全性初步评价

Primary safety evaluation of a novel freeze dried bone

目的:对一种新型掺锶冻干骨材料的安全性进行评价。方法:用离子交换方法制备掺锶冻干骨材料,制备浸提液,选择L929细胞通过MTT方法进行细胞毒性检验;应用小鼠进行体内植入,观察材料的异常毒性。结果:本实验制备的掺锶冻干骨材料锶元素含量为5.14%,细胞毒性评级为安全无毒级,小鼠体内实验未见异常毒性。结论:新型掺锶冻干骨材料是一种较为安全的新型骨替代材料。

Objective：To evaluate the safety of a novel Sr-incorporated freeze dried bone. Method： Ion exchange method was adopted to prepare Sr-incorporated freeze dried bone.After extraction, L929 cell was used to evaluate cytotoxicity by MTT method. The material was implanted into mouse and abnormal toxicity was then assayed. Result： The Sr density of the novel material is 5.14 %. Cytotoxicity of the material is ranked to nontoxic class and no abnormal toxicity effect was observed. Conclusion： Sr-incorporated freeze dried bone is a comparatively safe novel bone substitute.