间隙连接蛋白43在口腔颌面部鳞癌组织及舌癌细胞株(Tca8113)中表达的研究

The study of the expression of the Cx43 in the oral squamous cell carcinoma and Tca8113

目的:探讨间隙连接蛋白43(Cx43)在口腔鳞癌组织(oscc)和舌鳞癌细胞株(Tca8113)中的表达及其生物学行为的关系;细胞分化诱导剂全反式维甲酸(ATRA)对人舌鳞癌细胞株(Tca8113)中Cx43表达的影响。方法:应用免疫组织、细胞化学、免疫荧光及结合免疫印迹技术,观察OSCC组织中以及ATRA对Tca8113细胞株进行培养及分化诱导后Cx43在蛋白水平的变化。结果:Cx43在正常口腔鳞状上皮主要表达于相邻细胞间相互接触的细胞膜上,而在OSCC组织中,Cx43细胞膜表达与组织的分化程度、转移之间有显著性差异(Cx43:х2=7.42、12.43,P<0.05、P<0.01)。免疫细胞化学检测可见舌癌细胞中Cx43异常定位于细胞浆中和/或细胞膜不与邻近细胞接触的游离缘上。Tca8113经ATRA诱导后,Cx43蛋白表达增加。结论:Cx43表达的改变与OSCC的发生和发展有关。ATRA可以通过上调Cx43表达。

Objective： To investigate the expression of connexin 43 and its relationship with the biological behavior in oral squamous cell carcinoma （OSCC） and Tca8113; to investigate the effects of connexin 43 in tongue carcinoma cell line （Tca8113） by all-trans-retinoic acid （ATRA）. Method-Immunohistochemical and immunofluorescence was used to detect the expressions of Cx43 in oral squamous carcinoma. Western blot was adopted to examine the expressions of Cx43 in tongue carcinoma cell line （Tea8113） by all-trans-retinoic acid （ATRA）. Result-In normal oral squamous epithelium the expres- sion of Cx43 was typically shown in the membrane at intercellular contact, but in OSCC they were aberrantly expressed in cytoplasm.The significant difference was observed between low Cx43 expression and differentiation,metastasis （Cx43：x^2= 7.42, 12.43,P 〈0.05,P 〈0.01）.In Tca8113 cell line Cx43 was detected mainly either intracytoplas-mically or in plasma membrane free from contact with other cells by immunocytochemistry and immunofluorescence. After treated by ATRA, the expressions of Cx43 were increased in Tca8113. Conclusion： The data suggest that quantitative and qualitative changes of connexin43 expression are associated with progression and differentiation of human oral squamous cell carcinoma. ATRT can achieve up-regulation of Cx43, which should be responsible for inhibited of tumor cell growth.