牡荆素血浆蛋白结合率的测定

Determination of plasma protein binding rate of vitexin

目的:建立血浆中牡荆素浓度的分析方法,测定牡荆素血浆蛋白结合率。方法:采用平衡透析法透析,利用HPLC测定血浆中牡荆素浓度,计算牡荆素与人血浆蛋白结合率,并比较大鼠和人血浆中牡荆素的血浆蛋白结合率。结果:在2～16 mg.L-1,药物浓度对血浆蛋白结合率无显著影响,但牡荆素在大鼠和人的血浆蛋白结合率存在显著性差异,牡荆素与人的血浆蛋白结合率高于与大鼠血浆蛋白结合率。结论:牡荆素与血浆蛋白具有较强的结合。

Objective： To establish an analytical method on vitexin concentration in plasma to determine the plasma protein binding rate of vitexin. Method ： The equilibrium dialysis method and HPLC were adopted to determine vitexin concentration in plasma, calculate human plasma protein binding rate of vitexin and compare rat and human plasma protein binding rates of vitexin. Result ： At 2-16 mg · L^-1 , there was no significant difference in the plasma protein binding rate. But the hmnan plasma protein binding rate of vitexin was higher than its rat plasma protein binding rate, indicating a significant difference in rat and human plasma protein binding rates of vitexin. Conclusion ： Vitexin has a higher protein binding rate with both rat plasma and human plasma.