气相色谱法测定水果和蔬菜中5种有机含磷农药的残留量

GC Determination of Residual Amounts of 5 Organophosphorus Pesticides in Fruits and Vegetables

提出了用气相色谱-火焰光度检测器测定水果和蔬菜中甲基异柳磷、苯线磷、内吸磷、硫环磷和蝇毒磷等5种有机含磷农药残留量的方法。采用乙腈匀质提取样品中残留的有机含磷农药,提取液经石墨化炭黑粉末净化。用SPB-608毛细管色谱柱分离,气相色谱-火焰光度检测器法测定。方法的检出限(3S/N)在0.01～0.05mg·L-1之间。所测5种有机含磷农药的标准加入回收率在91.3%～110.0%之间,相对标准偏差(n=6)在1.5%～4.6%之间。

The residual amounts of five organophosphorus pesticides, including isofenphos-methyl, fenamiphos, demeton, phosfolan and＇ coumaphos, in fruits and vegetables were determined by gas chromatography with flame photometric detector （GC-FPD）. The sample was extracted homogeneously with acetontrile, and the extract was purified with graphitized carbon black. SPB-608 eapillary column was used for separation, and FPD was used for determination. Detection limits （3S/N） for the 5 organophosphorus pesticicdes found were in the range of 0. 01-0. 05 mg . L^-1. Recovery and precision were tested and results of recovery obtained by standard addition method were in the range of 91.3%-110. 0%, and values of RSD＇s （n=6） were in the range of 1.5%--4.6%.