黄芩苷对结核分枝杆菌抑菌作用的初步研究

Study of Antituberculosis of Baicalin on Mycobacterium Tuberculosis

目的:通过体外抑菌试验探讨黄芩苷对结核分枝杆菌的体外抑菌活性。方法:采用改良罗氏培养基绝对浓度法检测黄芩苷对37株结核分枝杆菌的抑菌作用。结果:黄芩苷对37株结核分枝杆菌临床分离株的最低抑菌浓度(MIC)最低为1.5g/L,最高>48g/L,MIC90为12g/L,MIC75和MIC50均为6g/L。全敏感菌株在MIC为6g/L时数量比例最大,随着MIC值向两侧递减;异烟肼(INH)低耐菌株的数量与MIC值无明显关联,乙胺丁醇(EMB)低耐菌株数量随着MIC由低到高的变化而显著增多;利福平(RFP)低耐菌株数量随着MIC由低到高的变化而逐步减少。结论:黄芩苷在体外对结核分枝杆菌具有一定的抑菌作用。

To analyze the antituberculosis of baicalin on mycobacterium tuberculosis by antimicrobial sus- ceptibility testing in vitro. Methods： The bacteriostatic action of baicalin on mycobacterium tuberculosis was detected by ab- solute consistence method of Lowenstein-Jensen. Results： The minimum inhibitory concentration （MIC） of baicalin on 37 strains of myeobacterlum tuberculosis was 1.5 g/L, and the maximum inhibitory concentration was 〉 48 g/L. MIC90 was 12 g/L. MIC75 and MIC50 were 6 g/L. The number of drug sensitive mycobacterium tuberculosis was most in 6 g/L MIC, and de- creased progressively with MIC value changed to both sides of the descending. There was not relevance between the strain number of mild drug resistance to mycobacterium tuberculosis and the MIC value. The number of ethambutal （EMB） mild re- sistance strain was increased progressively with MIC value changed from low to high. The number of rifampicin （RFP） mild re- sistance strain was decreased progressively with MIC changed from low to high. Conclusion： There is antitubereulosis of ba- icalin on mycobacterium tuberculosis in vitra.