表皮生长因子联合自体血浆冷沉淀对百草枯致角膜损伤的疗效

Therapeutic Effects of epidermal growth factor （EGF） combined with plasma cryoprecipitate （CRYO）on the corneal injury induced by paraquat

目的评价表皮生长因子（epidermal growth factor，EGF）、血浆冷沉淀（plasma cryoprecipitate，CRYO）以及EGF联合CRYO对百草枯致角膜损伤的疗效。方法参照《农药登记毒理学实验方法》将100斗l、20％的百草枯滴人健康白色新西兰家兔结膜囊内，构建百草枯致眼损伤的动物模型。18只百草枯致眼损伤动物模型构建成功后，随机分为3组（EGF治疗组：自体CORY治疗组和EGF＋GORY治疗组），每组6只，动物右眼接受冲洗和使用抗生素等常规治疗，左眼在常规治疗基础上分别接受EGF、CRYO和EGF＋CRYO治疗，观察结膜、角膜和虹膜的损伤情况和角膜荧光素滞留情况，计算眼损伤累加最高积分和角膜损伤恢复时间，对角膜损伤恢复情况进行病理组织学观察。结果EGF治疗组角膜损伤完全恢复时间为（19．50±3．08）d，低于常规治疗组[（27．33±2．58）d]，差异有统计学意义（P〈0．05）；EGF＋CRYO治疗组角膜损伤完全恢复时间为（18．67±2．73）d明显低于常规治疗组[（26．83±3．13）d]，差异有统计学意义（P〈0．05）。结论EGF以及ECG联合CRYO治疗百草枯致角膜损伤有效。

Objective To evaluate the therapeutic effects of epidermal growth factor （EGF） combined with plasma cryoprecipitate （CRYO） on the corneal injury induced by paraquat （PQ）. Methods According to the ＂Toxicological test methods of pesticides for registration＂ （GB 15670-1995）, the conjunctival sacs of 18 health New Zealand rabbits were exposed to 100μ1 20% PQ, which were randomly divided into EGF, CRYO and EGF plus CRYO groups. The routine treatments （normal saline washing and antibiotic eyedrops） were administrated to the injured eyes of 3 groups, at the same time the left eyes of 3 groups were treated with EGF, CRYO and EGF plus CRYO, respectively. The injury of conjunctival, iris and corneal, fluorescent stranded and pathology changes of corneal were observed. The injury score was calculated and the recovery time of corneal injury was recorded. Results The recovery time of corneal injury in EGF and EGF plus CRYO groups were 19.50±3.08 and 18.67±2.73 days, respectively which were significantly lower than those （27.33±2.58 and 26.83±3.13 days） in corresponding routine treatment controls （P〈0.05）. Conclusion EGF and EGF plus CRYO could be used to treat the corneal injury induced by paraquat.