rs9904341多态性与肺癌易感性和铂类药物化疗敏感性的相关性

The Relationship of rs9904341 Polymorphism with Lung Cancer Susceptibility and Sensitivity of Platinum-Based Chemotherapy in Patients with Lung Cancer

[目的]探讨survivin基因启动子区rs9904341位点多态性与肺癌发生风险和晚期非小细胞肺癌(NSCLC)患者铂类药物化疗疗效的关系。[方法]通过聚合酶链反应—限制性酶切片断多态性方法(PCR-RFLP)检测289例健康对照组和289例肺癌患者的rs9904341多态性的基因型;其中130例晚期NSCLC仅接受铂类药物化疗,分析经2个周期化疗后的疗效及基因型与铂类药物化疗疗效和生存期的关系。[结果]基因型CC、CG、GG在肺癌组的分布频率分别为29.07%、47.05%、23.87%,在对照组的分布频率分别为20.42%、51.9%、27.68%,两组无显著性差异(P=0.053)。肺癌组中C等位基因和CC基因型的分布均显著高于对照组(P<0.05)。rs9904341多态性与晚期NSCLC患者铂类药物化疗有效率无显著相关性,但与临床受益率相关,GG基因型携带者的临床受益率是CC基因型者的2.06倍(95%CI:0.71～5.97,P=0.184)。对289例肺癌患者生存分析显示,CC基因型肺癌患者较CG+GG基因型预后差(95%CI:1.11～2.6,P=0.015)。[结论]rs9904341多态性与肺癌的发病风险、预后及晚期NSCLC患者铂类药物化疗临床受益率相关。

[Purpose ] To investigate the relationship of survivin gene promoter rs9904341 polymorphism with the risk of lung cancer and the effect of platinum-based chemotherapy on advanced NSCLC. [Methods] The genotypes of rs9904341 polymorphism in 289 lung cancer patients and＇289 healthy person were analyzed by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）,method. In those lung cancer patients, 130 cases with advanced NSCLC only received platinum-based chemotherapy,the clinical respoilse was evaluated after 2 cycles,and the relationship of gene promoter polymorphism with susceptibility and survival of lung cancer was analyzed. [Results] The genotype frequencies for CC,CG,GG were 29.07% ,47.05% ,23.87% in lung cancer group,and 20.42% ,51.9% ,27.68% in control group respectively. No significant difference in the distribution of sl, rvivin genotype was found between the two groups（P=-0.053）. The frequencies of the C allele and CC genotype in lung cancer patients were significantly higher than those in healthy person （P〈0.05）. Analysis of 130 advanced NSCLC patients with platinum-based chemotherapy showed that no significant correlation of the rs9904341 polymorphism with response rate,but there is a rela- tionship with the clinical benefit rate. The clinical benefit rate in GG genotype was 2.06 times of the CC genotype （95%CI：071-5.97,P=0.184） with survival analysis for the 289 cases with lung cancer,compared with CG＋GG genotypes,the CC genotype was significantly associated with poor survival（95%CI：1.11-2.6,P=- 0.015）. [Conclusion ] The rs9904341 polymorphism is closely associated with susceptibility and prognosis of lung cancer,and is significant association with clinical benefit response in advance NSCLC receiving platinum-based chemotherapy.