蜈蚣药材薄层鉴别及抗凝活性定量的研究

Research on TLC Identification and Anti-coagulant Activity Quantitatively Methods of Scolopendra subspinipes mutilans

目的:对蜈蚣药材的薄层鉴别及抗凝活性定量方法进行研究,为该药材质量控制标准的完善提供依据。方法:对蜈蚣药材中游离精、丝氨酸进行薄层层析,筛选供试品制备方法及展开剂系统;以凝血酶滴定法测定蜈蚣药材抗凝活性,筛选药材前处理方法。结果:蜈蚣药材经甲酸及95%乙醇(1∶1)超声处理后,以正丁醇-乙酸-水(12∶5∶4)体系于硅胶G板上进行薄层层析,经茚三酮显色后,斑点清晰,杂质及拖尾影响较小,目标成分分离度较好;以凝血酶滴定法测定蜈蚣药材抗凝活性,结果重现性较好,超声法提取药材中的抗凝成分,操作简单,耗时较短,所制供试品抗凝血酶活性为(14.00±1.53)U/g;另测3批不同批次蜈蚣药材抗凝活性分别为:(13.00±0.58)U/g、(17.00±1.15)U/g、(15.67±1.53)U/g。结论:所选择的薄层鉴别及抗凝活性定量方法可作为完善蜈蚣药材质量标准的依据。

Objective：To improve the quality standard of Scolopendra subspinipes mutilans by researching the methods of the TLC identification and anti-coagulant activity quantitatively. Methods：Identified the free arginine（Arg） and serine （Ser） in scolopendra by TLC, screened the samples preparation process and developed solvent systems ; Determined the anti-coagulant activity by method of ti- tration with thrombin and screened the pretreatment methods. Results ：When medicinal materials was extracted by formic acid and 95% ethanol（ 1：1 ） with ultrasonic method and developed by n-butanol-acetic acid-water（ 12： 5： 4）, the spots of Arg and Ser were well sepa- rated. Ultrasonic method was suitable for preparation of the anti-coagulant components in Scolopendra subspinipes mutilans and their an- ti-coagulant activity was determined by method of titration with thrombin could get a well reproducibility, the anti-thrombin activity of testing sample yeas （ 14.130 ± 1.53 ） U/g and those of three different hatch were （ 13.00 ± 0.58 ） U/g, （ t 7.00 ± t. t 5 ） U/g, （ 1 5.67 ± 1.53 ）U/g respectively. Conclusion：The methods of TLC identification and anti-coagulant activity quantitatively could be used as a ba-sis for improving the quality standard of Scolopendra subspinipes mutilans.