不同加工方法对亳菊黄酮类成分含量影响

The Influence of Different Processing on the Content of Flavones of Dendranthema morifolium

目的:通过测定亳菊中木犀草素-7-O-β-D-葡萄糖苷、金合欢素-7-O-β-D-葡萄糖苷、木犀草素和金合欢素的含量,考察加工方法对质量的影响。方法:采用phenomenex,synergi Fusion-RP色谱柱(5μm,4.6 mm×250 mm),0.1%磷酸水-甲醇梯度洗脱,检测波长350 nm。结果:不同加工方法对亳菊中木犀草素-7-O-β-D-葡萄糖苷、金合欢素-7-O-β-D-葡萄糖苷、木犀草素和金合欢素含量有明显的影响。结论:不同加工方法对亳菊的黄酮类成分有较大影响,产地加工时应注意烘干的温度。

Objective ： To investigate the effect of different processing methods on the content of luteolin-7-O-β-D-glucoside, acace-tin-7-O-β-D-glucoside, luteolin, and acacetin of Dendranthema morifolium. Methods： The optimal conditions were achieved on phe-nomenex, synergi Fusion-RP（5 μm, 4. 6 mm ×250 mm）analytical column with a gradient mobile phase consisting of methanol and 0. 1% phosphoric acid and detection wavelength set at 350 nm. Results： The significant effect of different processing methods on the content of luteolin-7-O-β-D-glucoside, acaeetin-7-O-β-D-glucoside, luteolin and acacetin was proved. Conclusion： Different processing methods have significant effect on flavonoids of Dendranthema morifolium, it should be paid attention to drying temperature while sul-phur fumigation.