硫磺熏蒸前后菊花的HPLC指纹图谱比较研究

Study on HPLC Fingerprints of Chrysanthemi Flos before and after Sulfur-fumigation

目的:建立菊花药材指纹图谱检测方法,并对硫磺熏蒸前后菊花药材的指纹图谱进行分析比较。方法:采用高效液相色谱法,色谱柱为Waters C18(250 mm×4.6 mm,5μm);流动相为乙腈-0.5%磷酸水溶液,梯度洗脱;柱温35℃,检测波长268 nm。结果:测定未经硫磺熏蒸和经过硫磺熏蒸的菊花药材样品共11批,建立了菊花药材HPLC指纹图谱的共有模式,确立12个共有峰,并且采用聚类分析方法对11批样品进行分析比较。结论:该方法准确可靠,重复性好,可用于硫磺熏蒸前后菊花药材的质量评价与控制。

Objective：To evaluate the quality of Chrysanthemi Flos before and after sulfur-fumigation by analysis of HPLC finger-prints. Methods：RP-HPLC method was applied to establish the chromatographic fingerprint. The separation was performed on a Waters Cls （250 mm × 4. 6 mm ,5 μm）column with a gradient elution composed of acetonitrile-aqueous solution containing 0. 5% phosphoric acid. The column temperature was 35 ℃, the detective wavelength was set at 268 nm Results：Eleven batches of Chrysanthemi Flos samples composed of both sun-dried and sulfur-fumigated were analyzed and evaluated respectively. The method of clustering analysis was used, and the chromatographic fingerprint common pattern was finally established. Conclusion：The HPLC fingerprint method is reli-able and suitable for quality evaluation of Chrysanthemi Flos before and after sulfur-fumigation.