基于化学计量学的左金丸及其类方组分溶出规律的模式识别研究

Research on Pattern Recognition of the Component Stripping Rule Based on Zuojinwan and Its Similar Formulaes Combined Chemometrics Methods

目的:建立左金丸及其类方的HPLC相对溶出率图谱,对其溶出规律进行化学模式识别和验证。方法:以左金丸及其类方HPLC图谱中各组分的相对溶出率为指标,建立左金丸及其类方HPLC相对溶出率图谱,并使用主成分分析、系统聚类分析和Fisher判别分析对其溶出规律进行模式识别研究。结果:主成分分析划分的3个主成分可全面概括左金丸及其类方HPLC相对溶出率图谱的20项指标;在主成分分析的基础上,采用聚类分析将左金丸及其类方各组分溶出规律划为6类,并建立了相应的Fisher判别函数,回判准确率100%。结论:初步建立了左金丸及其类方各组分溶出规律的化学模式识别的评价方法,可对左金丸及其类方的溶出规律进行准确、可靠、客观的识别和验证。

Objective：To establish a method of HPLC relative dissolution rate for reorganizing and validating the dissolution pattern of Zuojinwan and its similar formulaes. Methods ： Selected the relative dissolution rate of the components of HPLC chromatogram of Zuo-jinwan and its similar formulaes as the index, establish a HPLC relative dissolution rate of Zuojinwan and its similar formulaes, and made the pattern recognition research on the dissolution of pattern based on using the principal component analysis, cluster analysis and Fisher discriminant analysis. Results：Three principal components divided from principal component analysis could summary compre- hensively the twenty indicators of HPLC relative dissolution rate of Zuojinwan and its similar formulaes ; Basing on the principal compo-nent analysis, divided the components dissolved rule of Zuojinwan and its similar formulaes into six classes through cluster analysis, and established the corresponding Fisher discriminant function,which return sentence accuracy rate was 100%. Conclusion ：The evaluation method established preliminarily of components stripping rules of chemical pattern recognition of Zuojinwan and its similar formulaes, can make the accurate, reliable, objective identification and validation of the stripping rule of Zuojinwan and its similar formulaes.