急性有氧运动对糖尿病大鼠肝脏和胰腺内质网应激蛋白和血糖的影响

Acute Aerobic Exercise Affects Liver and Pancreas Endoplasmic Reticulum Stress Protein and Blood Glucose of Diabetic Rats

目的:探讨急性有氧运动对糖尿病大鼠肝脏和胰腺内质网应激蛋白和血糖的影响,为糖尿病的运动疗法提供参考。方法:SPF级雄性SD大鼠55只,高脂饲料喂养配合腹腔注射小剂量链脲佐菌素制备2型糖尿病大鼠动物模型后,随机分为安静组(DM)和大、中、小三个强度有氧运动组(HIE-1、MIE-1、LIE-1),三个有氧运动组进行急性跑台运动,其运动速度分别为20 m/min、15 m/min和10 m/min,相当于70%VO2max、50%VO2max和30%VO2max负荷强度,持续运动1 h。运动后即刻检测空腹血糖(FBG)、糖化血清蛋白(GSP)、空腹胰岛素(Ins)、肝脏和胰腺的GRP78和caspase-12含量,并计算胰岛素抵抗指数(IRI)、胰岛素敏感指数(ISI)和β细胞功能指数(HBCI)。结果:各运动组糖尿病大鼠急性运动后血清空腹胰岛素较安静组均显著下降(P<0.01,P<0.01,P<0.05),胰岛素抵抗指数显著降低,胰岛素敏感指数显著升高,其中,大、中强度运动组变化有统计学意义(P<0.01)。中、小强度运动组肝脏和胰腺内质网应激蛋白GRP78和caspase-12较安静组均显著降低。结论:急性有氧耐力运动可降低糖尿病大鼠血糖水平,增强胰岛素敏感性,降低内质网过度应激状态,其机制可能与内质网应激蛋白参与调节有关。

Objective To investigate the effect of acute aerobic exercise on hepatic and pancreatic endoplasmic reticulum stress protein and blood glucose of diabetic rats. Methods Type 2 diabetes rat model was established by feeding high-fat diet and injecting low-dose streptozotocin intraperitoneally. Rats in the model were randomly divided into the control group （DM）,and 3 exercise groups （HIE-l, MIE-1 ,LIE-l）. After adapting to treadmill exercise,rats in groups LIE-1 ,MIE-1 ,and HIE-1 respec- tively underwent lh treadmill running at the speeds of 20 ndmin, 15 ndmin and 10 ndmin （equivalent to 70% VO2max, 50% VO2max and 30% VO2max）. Immediately after exercise, fasting blood glucose （FBG）, saccharifyed serum protein （GSP）, fasting insulin （Fins）, GRP78 and caspase- 12 contents in liver and pancreas were tested, then insulin sensitivity index （ISI）,insulin resistance index （IRI）, and Homa beta cell function index （HBCI） were calculated. Results Compared with group DM,after acute single exercise, the Fins of three exercise groups decreased significantly （P 〈 0.01 ,P 〈 0.01 ,P 〈 0.05） ,as well asIRI; and ISI increased significantly with significant differences among the three exercise groups （P 〈 0.01 ）. As compared with group DM ,the GRP78 and caspased-12 in liver and pancreas of groups MIE-1 and LIE-1 decreased significantly （P 〈 0.01 ,P 〈 0.05 ,P 〈 0.01 ）. Conclusion Acute aerobic endurance exercise can reduce blood glucose levels, increase insulin sensitivity and decrease the endoplasmic reticulum stress. Lower level of blood sugar may be related to the involvement of endoplasmic reticulum stress protein in regulation.