超声辐射微泡配合内皮祖细胞移植治疗糖尿病大鼠阴茎勃起功能障碍

Ultrasonic microbubbles destruction combining endothelial progenitor cells for improvement of erectile function in rats with diabetic erectile dysfunction

目的探讨超声微泡配合内皮祖细胞(EPCs)移植至海绵体组织治疗大鼠糖尿病阴茎勃起功能障碍的可行性。方法体外培养、分离EPCs。将54只成功建立糖尿病勃起功能障碍模型的SD大鼠随机分为空白对照组、EPCs治疗组、1.0W/cm2超声+微泡+EPCs组(US+MB+EPCs治疗组)。EPCs移植7天后,以脱水吗啡(APO)诱导实验检测大鼠阴茎勃起次数和勃起率,组织学观察阴茎海绵体,计数毛细血管数目,计算血管密度,免疫组化检测EPCs移植大鼠阴茎海绵体的情况,Western blot检测VEGF蛋白的表达。结果 US+MB+EPCs治疗组勃起次数和勃起率、毛细血管密度、EPCs染色强度的阳性指数均高于其他两组(P均<0.05),且VEGF蛋白表达水平最高(P<0.05)。结论超声微泡联合EPCs移植可提高EPCs的转染率和靶向性,改善糖尿病大鼠的阴茎勃起功能。

Objective To explore the feasibility of improving the delivery of endothelial progenitor ceils （EPCs） to caver- nosum tissue for the treating rat diabetes-induced erectile dysfunction by using ultrasonic microbubble destruction. Methods EPCs were derived from mouse bone marrow in vitro. Fifty-four SD rats were successfully induced into models of diabe- tes mellitus induced erectile dysfunction （DMED） and were randomly divided into 3 groups, i.e. control group, EPCs group and 1.0 W/cm^2 ultrasound＋microbubbles＋EPCs （US＋MB＋EPCs） group. All the rats were sacrificed 7 days af- ter EPCs implantation. The number of penile erections and erection rate were induced by apomorphine （APO）, and the vas- cular density of 3 groups was recorded after HE staining. EPCs expression was detected using immunohistochemistry, and VEGF protein was respectively detected with Western blot. Results Penile erections, erection rate, vascular density and EPCs expression were the best in US＋MB＋EPCs group （P〈0.05）. And the expression of VEGF protein in US＋MB＋ EPCs group was higher than that in two other groups （all P〈0.05）. Conclusion Combination of ultrasound microbubbles and EPCs can enhance the transfection efficiency and targeting of EPCs effectively, therefore improve the erectile function of DMED rats.