反复自然流产患者抗β_2糖蛋白I抗体、抗心磷脂抗体、封闭抗体检测及意义

Detection of anti-β2GPI,ACA and APLA in patients with recurrent spontaneous abortion and its significance

目的检测反复自然流产(recurrent spontaneous abortion,RSA)患者血清抗β2糖蛋白I抗体(抗β2-GPI抗体)、抗心磷脂抗体(anticardiolipin,ACA)及封闭抗体(antiphospholipid antibody,APLA)水平探讨其与RSA的关系。方法采用酶联免疫吸附试验(ELISA)分别对114例RSA患者和63例健康中期妊娠妇女血清测定抗β2-GPI抗体、ACA和APLA。结果 RSA患者血清抗β2-GPI抗体、ACA及APLA的阳性百分率分别为36%(42/114)、33.3%(38/114)和9.6%(11/114);健康对照组分别为3.0%(2/63)、4.8%(3/63)和31.7%(20/63)。RSA患者血清抗β2-GPI抗体、ACA明显高于对照组(P<0.05),APLA显著低于对照组(P<0.05)。结论血清抗β2-GPI抗体、ACA及APLA检测有助于不明原因RSA的病因诊断,与RSA发病有密切关系。

Objective To detect the serum level of anti-β2-Glycoprotein I antibodies（anti-β2GPI）,anticardiolipin antibodies（ACA） and anti-patemal lymphocyte antibody（APLA） in patients with recurrent spontaneous abortion（RSA） and to explore its relationship with RSA.Methods Enzyme-linked immunosorbent assay（ELISA） was used to detect the serum level of anti-β2GPI,ACA and APLA in both 114 patients with RSA and 63 healthy mid-term pregnant women.Results The positive percentage of anti-β2GPI,ACA and APLA in patients with RSA was 36%（42 /114）,33.3%（38 /114） and 9.6%（11 /114） respectively,while it was 3%（2 /63）,4.8%（3 /63）,and 31%（20 /63） in the healthy controls.The serum level of anti-β2GPI,ACA in patients with RSA was significantly higher than that in the control group（P 0.05） and APLA was significantly lower than that of the control group（P 0.05）.Conclusions Detection of anti-β2GPI,ACA and APLA contributes to the etiological diagnosis of unexplained RAS,for they are closely related to RSA.