地塞米松预处理人乳腺癌MCF-7细胞对多西他赛抗肿瘤活性的影响

Anti-tumor activity of docetaxel in human breast cancer cell line MCF-7 pretreated by dexamethasone

目的探讨地塞米松预处理人乳腺癌MCF-7细胞后,对化疗药物多西他赛(艾素)抗肿瘤活性的影响。方法以不同浓度(1×10-8～1×10-6 mol.L-1)的地塞米松预先作用于人乳腺癌MCF-7细胞后,再以艾素(5×10-6 mol·L-1)处理,在以上药物作用的相应时间段,通过流式细胞仪技术测定细胞凋亡,采用细胞计数观察细胞生长密度、形态。结果地塞米松对MCF-7细胞增殖有抑制作用(P<0.01),艾素能够明显抑制MCF-7细胞增殖,促进其凋亡(P<0.01),地塞米松预处理后艾素干预MCF-7细胞的增殖抑制作用较单用艾素减弱(P<0.01),且随地塞米松浓度的增加,其对化疗药艾素的凋亡抑制作用的影响增强。结论地塞米松预处理对艾素诱导的人乳腺癌MCF-7细胞的凋亡具有明显的阻抑作用,抑制了多西他赛的抗肿瘤活性。

Objective To investigate the antineoplastic activity of docetaxel in human breast cancer cell line MCF-7 pretreated by dexamethasone（Dex）.Methods With docetaxel contributed to the MCF-7 cells pretreated with dexamethasone at diverse concentration,flow cytometer was utilized to detect the cell apoptosis subsequently.The cells were counted and observed.Results Either Dex or docetaxel suppressed the generation of MCF-7 cells（P〈0.01）.The combined effect of the two drugs was attenuated,and the suppressive effect on the generation of docetaxel was lowered after Dex pretreatment（P〈0.01）.Conclusion The antineoplastic activity of docetaxel in human breast cancer cell line MCF-7 can be suppressed by dexamethasone.