糖尿病肾病中血管生成素2的表达及其与蛋白尿的相关性研究

Expression of angiopoietin-2 in patients with diabetes nephropathy and correlation study between anglopoietin-2 and proteinuria

目的探讨糖尿病肾病患者蛋白尿与血管生成素2（Ang-2）的相关性。方法回顾性分析104例糖尿病肾病患者临床资料，根据患者尿白蛋白排泄率（UAER）将其分为无蛋白尿组45例和蛋白尿组59例，采用放免法测定UAER，采用双抗体夹心酶联免疫吸附法测定血清Ang-2水平，同时测定患者血压、糖化血红蛋白（HbA，C）、空腹血糖（FBG）、三酰甘油（TG）、总胆固醇（TC）、肌酐（Cr）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）等实验室指标。结果两组舒张压、FBG、HbA，C、TG、TC、LDL-C、HDL-C比较差异无统计学意义（P〉0．05），蛋白尿组收缩压（140．1±17．4）mmHg（1mmHg=0．133kPa）、Cr（126．7±23．8）μmol/L，与无蛋白尿组的（126．3±15．2）mmHg、（70．3±22．1）μmol／L比较差异有统计学意义（P〈0．05）。蛋白尿组血清Ang-2为（24．86±4．31）ng／L，显著高于无蛋白尿组的（18．83±7．28）ng／L，两组比较差异有统计学意义（P〈0．01）。偏相关分析表明，UAER与血清Ang-2呈正相关（r=0．615，P〈0．01）。结论糖尿病肾病患者肾损害时，血清Ang-2水平显著升高。

Objective To investigate the correlation between proteinuria and anglopoietin-2 （Ang-2） in patients with diabetes nephropathy. Methods The clinical data of 104 patients with diabetes nephropathy were analyzed retrospectively. All the patients were divided into non-proteinuria group with 45 cases and protelnuria group with 59 cases according to the urinary albumin excretion rate （UAER）. UAER was detected by radioimmunoassay. The level of serum Ang-2 was detected by double antibody enzyme linked immunosorbent assay. Blood pressure, glycosylated hemoglobin （HbA1c）, fasting blood glucose （FBG）, treacylglycerol （TG）, total cholesterol（TC ）, creatinine （Cr）, low density lipoprotein cholesterol（LDL-C ）, high density lipoprotein cholesterol （HDL-C） were also detected. Results There was no significant difference in diastolic blood pressure, FBG, HbA1c, TG, TC, LDL-C, HDL-C between two groups （P 〉 0.05 ）. Systolic blood pressure and Cr in proteinuria group [（140.1±17.4） mm Hg （1 mm Hg =0.133 kPa）, （126.7±23.8） μ mol/L] had statistical significance compared with those in non-proteinuria group [ （ 126.3 ± 15.2） mm Hg, （70.3 ± 22.1 ） μ mol/L ] （P 〈 0.05）. The level of serum Ang-2 in proteinuria group was significantly higher than that in non-proteinuria group [（24.86 ±4.31） ng/L vs. （18.83 ±7.28） ng/L] （P 〈0.01）. Partial correlation analysis showed that UAER was positively correlated with Ang-2 （r =0.615,P 〈0.01）. Conclusions The level of serum Ang-2 increases significantly when there is renal damage in patients with diabetes nephropathy.