摘要
目的:建立复方南星止痛膏的顶空气相指纹图谱,全面评价其质量。方法:采用气相色谱法,色谱柱:HP-5MS(30m×0.25mm,0.25μm),载气为氮气,流速:1mL·min-1,分流比:5:1,FID检测器,程序升温,顶空进样。并对10批复方南星止痛膏挥发性成分指纹图谱进行相似度计算。结果:10批复方南星止痛膏指纹图谱中有6个共有峰,各峰分离度良好,样品间相似度均大于0.95。结论:本方法简便、准确、重现性好,可用于复方南星止痛膏的质量评价。
Objective:To establish a headspace GC fingerprint chromatogram for analysis volatile compounds of Fufang Nanxing Zhitong Gao, and evaluated its quality. Methods:The sample was conducted by GC with HP-5 column (30 m ×0. 25 mm, 0. 25 μm). The carrier gas was N2 at the flow rate of 1.0 mL. min - 1. Split ratio was 5 : 1. The detection was FID. Ten batches of Fufang Nanxing Zhitong Gao was calculated to their similarity. Results: Six main peaks were clearly separated in 20 min. The similarity of ten batches was over 0. 95. Conclusion:The headspace GC fingerprint chromatogram is successfully used on analysis and quality control of Fufang Nanxing Zhitong Gao.
出处
《中国现代中药》
CAS
2012年第7期49-51,共3页
Modern Chinese Medicine