大鼠心肌缺血再灌注后不同时间点心脏及肝脏中HIF-1、VEGF的表达

The expression of HIF-1 and VEGF in myocardium and liver after myocardial ischemia reperfusion injury

目的:探讨大鼠心肌缺血再灌注后不同时间点心脏和肝脏中缺氧诱导因子-1(hypoxia-inducible factor 1,HIF-1)及血管内皮生长因子(vascular endothelial growth factor,VEGF)的变化。方法:结扎雄性成年SD大鼠冠状动脉左前降支,缺血30min,再灌注6 h(I30minR6h)或24 h(I30minR24h),real-time PCR法检测心肌组织中HIF-1α和VEGF的mRNA水平,Western blot法检测心肌以及肝脏组织中HIF-1α、HIF-1β和VEGF蛋白表达的改变。结果:与假手术组相比,I30minR6h组心肌中HIF-1α和VEGFmRNA和蛋白水平明显增加,但I30minR24h组无明显变化。心肌组织中HIF-1β蛋白水平及肝脏中HIF-1α、HIF-1β和VEGF蛋白水平在不同再灌注时间点无明显改变。结论:心肌缺血再灌注影响心肌HIF-1和VEGF的表达,但对肝脏中HIF-1和VEGF的表达无影响,且其对心肌HIF-1和VEGF表达的影响与再灌注时间相关。

Objective：To study the expression of hypoxia-inducible factor 1（HIF-1） and vascular endothelial growth factor（VEGF） in myocardium and liver after myocardial ischemia reperfusion injury.Methods：Adult male SD rats were subjected to 30 min myocardial ischemia by ligation of the left anterior descending coronary artery and 6 h（I30minR6h） or 24h（I30minR24h） reperfusion.The protein expression of HIF-1α,HIF-1β and VEGF in myocardium and liver were examined by Western blot,and the mRNA levels of HIF-1α and VEGF were determined by real-time PCR.Results：Compared with sham group,both protein and mRNA levels of HIF-1α and VEGF were increased in the ischemic region of myocardium in I30minR6h,but unaffected in I30minR24h.The level of HIF-1β was not different in myocardium among the three groups.But compared with sham group,there were no differences of the protein expression of HIF-1α,HIF-1β or VEGF in liver after myocardial I30minR6h or I30minR24h.Conclusion：The expression of HIF-1α and VEGF in myocardium were affected in myocardial ischemia reperfusion injury whereas not changed in liver.The affection was associated with the time of reperfusion.