室间隔缺损膜部瘤组织内细胞增殖和生存能力分析

Proliferation and survival analysis of aneurysms of membranous septum associated with perimembranous ventricular septal defect

目的:通过对室间隔缺损合并的膜部瘤组织内细胞的增殖和存活能力进行组织学分析,了解膜部瘤这一特殊组织的病理学特性。方法:采用HE染色对假性和真性膜部瘤组织进行组织结构分析,采用Ki67和磷酸化Akt(pAkt)免疫组织化学染色对膜部瘤组织细胞的增殖和存活能力进行分析。结果:HE染色表明假性膜部瘤组织内细胞数量显著多于真性膜部瘤,但细胞外基质较少,结构比较疏松。Ki67染色结果提示假性膜部瘤细胞增殖能力强于真性膜部瘤,而磷酸化的Akt表达仅见于假性膜部瘤组织内部分细胞,真性膜部瘤组织无表达。结论:假性膜部瘤组织具有较强的细胞增殖和存活能力,是一种处于活跃状态的组织。

Objective：To explore the pathological characteristics of aneurysms of membranous septum（AMS） associated with perimembranous ventricular septal defect by analyzing cell proliferation and survival.Methods：The structure of AMS was analyzed by HE staining.Immunohistochemical staining of Ki67 and phosphorylated Akt（pAkt） was performed to identify cell proliferation and survival in AMS.Results：HE staining showed that the number of cells in false AMS was more than that in true AMS,but the extracellular matrices of false AMS were less compact than those of true AMS.Much more Ki67 positive cells in false AMS suggested a more powerful proliferation than true AMS.pAkt staining was only found in endothelial and subendothelial cells in false AMS.Conclusion：False AMS demonstrates stronger ability of cellular proliferation and survival indicating it’s an active tissue.