应用组织芯片技术检测结直肠癌中MRP1/CD9的表达

Detecting of MRP1/CD9 in colorectal carcinoma using tissue microarrays

目的:探讨结直肠癌组织中运动相关蛋白-1(MRP1/CD9)的表达及其与肿瘤转移的关系。方法:应用组织芯片和免疫组织化学技术检测244例结直肠癌组织中MRP1/CD9的蛋白表达。结果:MRP1/CD9在结直肠癌组织中阳性率为77.9%,其表达与患者的性别、年龄、浸润深度无关,而与肿瘤的分化程度、Dukes分期、淋巴结转移有关;其中高-中分化组表达高于低分化组;Dukes A+B期表达高于C+D组;无淋巴结转移组表达高于有淋巴转移组,差异均具有统计学意义(P<0.05)。二元Logistic回归前进法显示:分化程度最先进入方程,是影响MRP1/CD9表达最显著因素。MRP1/CD9表达在单因素时和Dukes分期负相关,但在多因素时被掩盖。结论:MRP1/CD9在结直肠癌组织中的表达缺失与肿瘤的转移密切相关,有望成为判断结直肠癌预后的新标志。

Objective：To investigate the expression of MRP1/CD9 as well as their correlations with the metastasis of colorectal carcinoma.Methods：Tissue microarray and immunohistochemistry were used to detect the expression of MRP1/CD9 in 244 cases of colorectal carcinoma.Results：The positive rate of MRP1/CD9 expression was 77.9% in colorectal carcinoma.The expression of MRP1/CD9 had no relationship with the patients’ sex,age,and the depth of infiltration,but it had relationships with the differentiated degree,Dukes stages,and lymphnode metastasis.The expression of MRP1/CD9 was higher in colorectal carcinomas with moderate or high differention than that in the ones with poor differention.The expression of MRP1/CD9 was higher in colorectal carcinomas with Dukes A＋B stages than that in the ones with Dukes C＋D stages.The expression of MRP1/CD9 was higher in colorectal carcinomas without lymphnode metastasis than that in the ones with lymphnode metastasis.Above all had the significant meaning（P 〈 0.05）.Forward stepwise binary logistic regression reveals：differentiation,which went into the equation first,is the significant factor of MRP1/CD9 expression.The expression of MRP1/CD9 was negatively related to Dukes stages in monofactoral analysis,but disappearing in multifactoral analysis.Conclusion：The loss expression of MRP1/CD9 are closed correlated with the metastasis of colorectal carcinoma,and this marker has hopes to become a new target to evaluate the tumor prognosis.