不同采收期青翘中4种活性成分的含量分析

Analysis of the contents of four active ingredients in Forsythiae fructus gathered in different periods

【目的】以不同采收期青翘中连翘苷、连翘酯苷A、芦丁和槲皮素的含量为指标,综合比较4种活性成分含量以确定青翘的最佳采收期。【方法】采用高效液相色谱(HPLC)法,测定不同采收期采自陕西省洛南县的青翘中连翘苷、连翘酯苷A、芦丁、槲皮素的含量,并对这4种活性成分的含量进行比较分析。【结果】7月下旬至8月上旬青翘中的连翘苷、连翘酯苷A、芦丁的含量较高,最高分别可达5.62,41.92,5.70mg/g;这3种活性物质含量随采收时间的延长而波动较大。槲皮素含量以7月下旬至9月上旬采收青翘较高,最高达0.91mg/g,且不同采收期青翘中槲皮素的含量变化较小。【结论】陕西省洛南县产青翘于7月下旬至8月上旬采收最佳,此时连翘苷、连翘酯苷A、芦丁和槲皮素的含量均较高。

【Objective】 This study evaluated the suitable gathering periods by comparing the contents of phillyrin,forsythiasideA,rutin and quercetin of Forsythiae fructus gathered in different periods.【Method】 HPLC method was used to assay the contents of phillyrin,forsythiasideA,rutin and quercetin.【Result】 The contents of phillyrin,forsythiasideA and rutin arrived at its maximum yield in late July and early August,reaching 5.62,41.92,5.70 mg/g respectively.The three kinds of material contents fluctuated much in the different recovery time.The content of quercetin arrived at its maximum yield in late July and early September（0.91 mg/g）,and changed little in the different recovery time.【Conclusion】 The contents of phillyrin,forsythiasideA,rutin and quercetin are higher in late July and early August.