萘降解菌MQ合成靛蓝的研究

Biosynthesis of indigo by naphthalene-degrading strain Comamonas sp.MQ

利用萘降解菌(丛毛单胞菌)MQ完整细胞对吲哚进行转化合成靛蓝,结果表明菌株MQ具有较好的靛蓝合成能力,靛蓝产量在4h左右即达到稳定.薄层色谱和高效液相色谱分析结果表明,合成的蓝色产物为靛蓝.考察了反应温度及摇床转速对靛蓝合成的影响,结果表明最适温度和转速分别为25℃和150r·min-1.随后,采用表面响应法确定了菌株MQ合成靛蓝的最优条件:菌株接种量OD6602.16,吲哚浓度200.55mg·L-1,pH=6.91.在最优条件下,菌株MQ合成靛蓝的产量为53.05mg·L-1,比初始条件下的产量提高了179%.

The whole cells of naphthalene-degrading strain Comamonas sp.MQ were used for the transformation of indole to indigo.The results showed that strain MQ possessed excellent activities for indigo production,and the maximum yield of indigo could be obtained within 4 h.Based on the analysis of thin-layer chromatography（TLC） and high performance liquid chromatography（HPLC）,the blue pigment was proved to be indigo.The effects of temperature and rotational speed on indigo production were investigated,and 25 ℃ and 150 r·min-1 were selected as the most favorable conditions.Other variables during indigo production were optimized by response surface methodology as follows：inoculum 2.16（OD660）,indole 200.55 mg·L-1 and pH 6.91.As a result,the yield of indigo obtained was up to 53.05 mg·L-1 under the optimal conditions,which was 179% higher than that of original conditions.