HPLC测定核桃青皮中胡桃醌的含量

Determination of Juglone in the Husk of Walnut by HPLC

目的:建立一种测定核桃青皮中胡桃醌含量的高效液相色谱法,为实现核桃青皮的合理开发利用提供依据。方法:采用高效液相色谱法测定了核桃鲜皮和冷冻青皮中的胡桃醌含量,对此方法进行了方法学评价。结果:胡桃醌对照品在0.01～8.00μg线性相关良好(r=0.999 3);检出限为0.195 ng;平均回收率为98.67%(n=6),RSD<2.03%;精密度RSD<0.58%(n=7)。新鲜核桃青皮中胡桃醌的含量为1.66 mg·g-1,冷冻青皮含量为1.54 mg·g-1。结论:该方法样品前处理简单、分析快速,重复性好,准确度和精密度高,适合胡桃醌类样品的分析测定。

Objective：To establish an HPLC method for determining the content of juglone in the husk of walnut,to provide the basis for the resource exploitation and utilization of juglone.Method：The method was evaluated by HPLC in methodology and the samples which were fresh and stored in freeze temperature were determined.Result： This HPLC methord have a simple preparation for popularization easily.The results indicate that this certain method had good linear relationship under the condition that the content was between 0.01 μg and 8 μg（r=0.999 3） and the detection limit of this method was 0.195 ng.The average recovery was 98.67%（n=6）,RSD was below 2.03%;the RSD of the indicated accuracy was below 0.58%（n=7）.Juglone in the husk of fresh walnut was 1.66 mg·g-1 and another sample was 1.54 mg·g-1 which was stored in freeze temperature.Conclusion： This certain method is simple,fast and reproducible with a high accuracy and precision,which fit for the analysis of juglone.