HPLC测定七归滴丸中阿魏酸、三七皂苷R_1、人参皂苷Rg_1、人参皂苷Rb_1的含量

Determination of Notoginsenoside R_1,Ginsenoside Rg_1 and Rb_1 in Qigui Drop Pill by HPLC

目的:建立测定七归滴丸中阿魏酸、三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量测定方法。方法:采用C18柱(4.6 mm×150 mm,5μm),流动相为甲醇-0.6%冰醋酸(30∶70),检测波长为323 nm,测定阿魏酸;采用C18柱(4.6 mm×250 mm,5μm),乙腈-水梯度洗脱,流速0.8 mL·min-1,检测波长203 nm,测定三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1。结果:阿魏酸的回收率为99.18%(RSD 1.4%);三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的回收率分别为98.77%(RSD1.71%),98.94%(RSD 1.58%),99.48%(RSD 1.65%)。结论:此法准确、可靠,重复性好,可用于控制七归滴丸的质量。

Objective： To develop a method for the determination of ferulic acid,notoginsenoside R1,ginsenoside Rg1 and Rb1 in Qigui drop pill by HPLC.Method： The content of ferulic acid was quantified by HPLC.A C18 column was used with the mobile phase of methanol-0.6% glacial acetic acid solution（30∶ 70）,at the detection wavelength of 323 nm.The content of sanchi notoginsenoside R1,ginsenoside Rg1 and Rb1 was quantified by HPLC.A C18 column was used with the mobile phase of acetonitrile-water gradient elution.Equilibrium was 0.8 min and the wavelength of detector was set at 203 nm.Result： The average recovery of ferulic acid was 99.18%（RSD 1.4%）.The average recoveries of notoginsenoside R1,ginsenoside Rg1 and Rb1 were 98.77%（RSD 1.71%）,98.94%（RSD 1.58%） and 99.48%（RSD 1.65%）.Conclusion： The method is accurate,reliable and reproducible.It can be used for the quality control of Qigui drop pill.