麻黄-甘草药对的抗炎作用及机制研究

Anti-inflammatory Effects and Mechanism of Ephedra sinica-Glycyrrhiza uralensis Herbal Compatibility

目的:观察麻黄-甘草药对的抗炎作用,探讨抗炎机制。方法:ICR小鼠随机分为模型对照组,地塞米松组(0.01g·kg-1),麻黄-甘草药对组(22.4,11.2,5.6 g·kg-1),每天1次,连续ig给药5 d,末次给药30 min后采用醋酸致腹腔毛细血管通透性增加实验观察抗炎作用;取SD大鼠随机分为正常对照组,模型对照组,地塞米松组(0.01 g·kg-1),麻黄-甘草药对组(22.4,11.2,5.6 g·kg-1),每天1次,连续ig给药5 d,末次给药30 min后采用角叉菜胶诱导胸膜炎实验观察抗炎作用,测定胸膜炎大鼠胸腔渗出液前列腺素E2(PGE2)、肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)含量以及肺组织TNF-α,IL-1β和丙二醛(MDA)含量,探讨抗炎机制。结果:与模型对照组比较,麻黄-甘草药对(22.4,11.2 g·kg-1)可显著抑制醋酸致小鼠腹腔毛细血管通透性增加,抑制角叉菜胶诱导的胸膜炎大鼠胸腔液渗出(P<0.01),抑制渗出液白细胞数量增多(P<0.01);22.4 g·kg-1能抑制中性粒细胞比率升高;麻黄-甘草药对3个剂量组对淋巴细胞比率没有明显影响;麻黄-甘草药对(22.4,11.2 g·kg-1)抑制胸腔液PGE2,TNF-α,IL-1β含量升高(P<0.05);药对22.4 g·kg-1能抑制肺组织TNF-α,IL-1β,MDA含量升高(P<0.01),11.2 g·kg-1能抑制肺组织IL-1β含量升高(P<0.01)。结论:麻黄-甘草药对有抗炎作用,抗炎机制可能与影响炎症介质产生和抗氧化作用有关。

Objective： To observe the anti-inflammatory effects of Ephedra sinica-Glycyrrhiza uralensis herbal compatibility and explore its mechanism.Method： ICR mice were divided into 5 groups randomly,namely the model group,dexameathone group（0.01 g·kg^-1）,E.sinica-G.uralensis herbal compatibility groups（22.4,11.2,5.6 g·kg^-1）.Each drug was administrated to mice orally once a day for 5 days.After 30 min of the last administration,anti-inflammatory effect was observed by the method of acetic acid-induced permeability accentuation.SD rats were divided into 6 groups randomly,namely the control group,model group,dexameathone group（0.01 g·kg^-1）,E.sinica-G.uralensis herbal compatibility groups（22.4,11.2,5.6 g·kg^-1）.Each drug was administrated to mice orally once a day for 5 days.After 30 min of the last administration,the rat model of carrageenan-induced acute pleurisy was also selected to observe the anti-inflamatory activity.The anti-inflammatory mechanisms were investigated by detecting the contents of prostaglandins（PGE2）,tumor necrosis factor-alpha（TNF-α）,interleukin-lβ（IL-1β） in exudates,and the contents of TNF-α,IL-1β,malondialdehyde（MDA） in lung tissue were also detected.Result： Compared with the model group,E.sinica-G.uralensisherbal compatibility（22.4,11.2 g·kg^-1）produced significant inhibitory effects on acetic acid-induced permeability accentuation in mice,and inhibited the increase of the volume of exudates（P0.01）,as well as the total leukocytes in the exudates（P0.01） induced by carrageenan,22.4 g·kg^-1 inhibited the increase of the percentage of neutrophil,and three doses of herbal compatibility had no significant effects on the percentage of lymphocytes,E.sinica-G.uralensis drug pair（22.4,11.2 g·kg^-1）significantly reduced the levels of PGE2,TNF-α,IL-1β in exudates（P0.05）,drug pair 22.4 g·kg^-1 also reduced the contents of TNF-α,IL-1β,MDA in lung tissue in carrageenan treated rats（P0.01）,11.2 g·kg^-1 reduced the contents of IL-1β in lung tissue（P0.01）.Conclusion： E.sinica-G.uralensis herbal compatibility possess significant anti-inflammatory effects,which can be related with the inhibition of the generation of mediators of inflammation and its antioxidation.