贵州产天冬醇提液体外氧自由基清除作用的研究

Antioxidative Activity of Alcoholic Extracts from Riadix Asparag

目的:研究贵州产天冬醇提液体外清除羟自由基(·OH)与超氧阴离子自由基(O2-)的作用。方法:选取贵州产天冬为研究对象,采用乙醇回流提取法提取其醇提液;采用连苯三酚法在碱性溶液中自氧化反应产生超氧阴离子自由基(O2-),采用邻二氮菲-Fe(Ⅱ)/H2O2体系产生羟自由基(·OH),研究天冬醇提液体外清除自由基的作用;结果:贵州产天冬醇提液对O2-具有显著的清除及抑制作用(P<0.05),而且随着药物浓度的增加,其清除及抑制作用也随之增加;贵州产天冬醇提液对·OH也有一定的清除作用(P<0.05),其清除率为(35.1±0.16)%。结论:贵州产天冬醇提液对O2-具有显著的清除及抑制作用,对·OH也有一定的清除作用。

Objective： To study the scavenging action of alcoholic extracts from Riadix Asparag on oxygen free radical.Method： The Asparagus ethanol-extraction liquid was estracted by ethanol refluxing extraction.Auto-oxidation in alkalcsent solution with pyrogallol can generate O2-and the system of 1,10-phenathroline-Fe（Ⅱ）/H2O2 canproduce·OH.Spectropholomemy was used to observe the effect of alcoholic extracts from Riadix Asparag upon O2-and·OH.Result：The alcoholic extracts from Riadix Asparag could decrease the levels of O2-and inhibit the generation of O2-in vitro;alcoholic extracts from Riadix Asparag could reduce the leve of ·OH（P0.05）.Inhibition ratio is 35.1%±0.16%.Conclusion：Guizhou alcoholic extracts from Riadix Asparag could scavenge and inhibit O2-markedly and scavenge·OH as well.