淫羊藿苷对体外培养大鼠股骨组织的影响

Effect of Icariin on Rats Femur Tissue in vitro

目的:研究淫羊藿苷对体外培养大鼠股骨组织的影响。方法:无菌条件下体外分离培养大鼠股骨组织,采用培养液中终浓度为1×10-5mol·L-1的淫羊藿苷进行药物干预,检测碱性磷酸酶活性(alkaline phosphatase,ALP)、钙盐沉积量以及Real-Time RT-PCR检测骨保护素(osteoprotegerin,OPG),Ⅰ-型胶原(collagen-1),Runt-related transcription factor 2(Runx-2)mRNA的表达情况。结果:1×10-5mol·L-1淫羊藿苷处理组ALP活性从第6～12天呈升高趋势,第12～18天降低;6～15 d各组钙盐沉积量呈升高趋势,第18天降低;淫羊蒮苷组处理股骨组织2～4 d时促进Runx-2 mRNA的表达水平,处理8 d时抑制Runx-2 mRNA的表达;淫羊蒮苷组处理股骨组织2～8 d时促进Collage-1 mRNA的表达水平;淫羊蒮苷组处理股骨组织2～4 d时能促进OPG mRNA的表达水平,处理6,8 d时与对照组比较无统计学意义。结论:淫羊藿苷可显著提高体外培养大鼠股骨组织中骨代谢相关基因的表达水平、增加钙盐沉积量以及ALP活性。

Objective： To investigate the effects of icariin on the femur tissue in vitro. Method： The rat femur tissue was isolated from SD rats with enzyme digestion. Icariin was supplemented into the culture at 1 × 10^-5mol·L^-1, alkaline phosphatase （ALP） activity and calcium content were compared between the icariin group and the control group. Total RNA was isolated and the gene expression of osteoprotegerin （OPG） , collagen-1, Rurt- related transcription factor 2 （Runx-2） was detected by Real-time RT-PCR. Result： 1 ×10^-5mol·L^-1 ieariin significantly promoted femur tissue metabolism related gene expression level, increased ALP activity and calcium content. Conclusion： Icariin can enhance the rat femur tissue in bone metabolism related gene expression, increase calcium content and ALP activity.