妇炎汤对急性盆腔炎大鼠血清炎性细胞因子表达的影响

Effect of Fuyan Decoction on Inflammatory Cytokines in Blood Serum of Acute Pelvic Inflammation Rats

目的:研究妇炎汤对急性盆腔炎大鼠血清炎性因子的影响。方法:采用金黄色葡萄球菌、解脲脲原体和大肠杆菌混合菌接种法建立急性盆腔炎模型。模型成功后随机分为假手术组、模型组、妇炎汤低、中、高剂量组、中药对照组(妇科千金片)。妇炎汤高、中、低剂量组分别以3.15,6.0,12.6 g·kg-1.d-1ig给药,连续给药20 d,末次给药后禁食12 h,取外周血,采用ELASA法检测白介素2(IL-2)、白介素6(IL-6)和肿瘤坏死因子α(TNF-α)的表达情况。并取卵巢和子宫组织,观察病理改变情况,同时,测血细胞的变化。结果:正常组IL-2,IL-6和TNF-α分别(117.62±3.24),(74.40±3.76),(22.17±2.87)ng·L-1;与正常组相比,模型组IL-2为(95.44±2.83)ng·L-1,明显降低(P<0.01);IL-6和TNF-α分别为(89.85±4.57),(33.48±4.52)ng·L-1,明显升高(P<0.01);与模型组相比,妇炎汤低、中剂量组IL-2,IL-6分别高于和低于模型组(P<0.05),低剂量组TNF-α则无显著性差异。高剂组IL-2高于模型组(P<0.01),IL-6和TNF-α低于模型组。治疗后,3组白细胞总数及中性粒细胞百分比均明显降低,子宫黏膜病理改变也明显减轻,炎细胞浸润明显减少。结论:妇炎汤对急性盆腔炎有一定防治作用,其机制可能与调节炎性细胞因子的表达有关。

Objective： To study the effect of Fuyan decoction on inflammatory cytokines in blood serum in acute pelvic inflammation rats.Method： The rat acute pelvic inflammation model was established by mixed bacteria of Staphylococcus aureus,Mycoplasma urealytium and Escherichia coli.Fuyan decoctions in high,middle,low dosage were used to treat acute pelvic inflammation rat for 20 days.The content of blood serum interleukin-2（IL-2）,interleukin-6（IL-6）,tumor necrosis factor-α（TNF-α） in acute pelvic inflammation rats was measured by enzyme-linked immunosorbent assay（ELISA）.Pathologic changes of oophoron and uterus were studied by microscope,in the same way,the number of blood corpuscle was measured by blood corpuscle analyzer.Result：The content of IL-2,IL-6 and TNF-α in normal group was（117.62±3.24）,（74.40±3.76）,（22.17±2.87）ng·L-1 respectively.In compare with normal group,the concentration of IL-2,IL-6 and TNF-α in model group was severally decreased and increased to（95.44±2.83）,（89.85±4.57）,（33.48±4.52） ng·L-1（P0.01）;IL-2 and IL-6 concentration in low and middle Fuyan decoction groups were respectively higher and lower than those in model group（P0.05）,TNF-α content was not different.Change of those in high dosage group was prominent（P0.01）;totality of white blood cell and percentage of neutrophile granulocyte were decreased obviously in three groups,Fuyan decoction could also abated the inflammation reaction of mucosa,lower number of inflammatory cell in mucosa.Conclusion： Fuyan decoction can prevent and treat acute pelvic inflammation by regulation of expression of inflammatory cytokines.