芪蚣抗纤方(QF)拆方中软坚散结药对免疫损伤性肝纤维化大鼠TGFβ-1定性定量表达的影响

Influence of Softening and Resolving Hard Mass herbs in Qigong kang xian formula on expression of TGF β-1 in rat model of liver fibrosis induced by immune damage

目的:观察芪蚣抗纤方(QF)拆方中软坚散结药(蜈蚣、山慈姑)对免疫损伤性肝纤维化大鼠TGFβ-1定性定量表达的影响。方法:猪血清攻击法诱导免疫损伤性肝纤维化大鼠模型,随机分为模型组、软坚散结药大剂量干预组、软坚散结药小剂量干预组,另设正常对照组,整个实验过程共8周。免疫组化法检测肝组织TGF-β1相关表达;酶标仪ABC-ELISA法测血清TGF-β1含量。结果:免疫组化显示:正常组大鼠肝脏仅TGFβ1微弱表达,模型组较正常组TGFβ1阳性信号明显增强(P<0.01),软坚散结药干预后大鼠肝脏TGFβ1表达比模型组明显下调,(小剂量P<0.01,大剂量P<0.05),大、小剂量组与模型组相比有差异。血清TGF-β1含量:与模型组相比,软坚散结药大、小剂量干预后动物血清中的TGFβl显著降低(P<0.01)。结论:芪蚣抗纤方(QF)中软坚散结药大、小剂量对免疫损伤性大鼠肝纤维化形成和逆转均有影响,对促肝纤维化细胞因子TGFβ-1均有很好调节作用,对临床上应用软坚散结药治疗肝纤维化有一定的指导意义。

Objective： To observe influence of Wugong and Shancigu in Qi ,Gong Kang Xian formu- la （QF） on expression of TGFβ-1 in rat model of liver fibrosis induced by immune damage. Methods： Rat models of liver fibrosis induced by immune damage were established with porcine serum attack method, and randomly divided into model group, high-dose group and low-dose group, healnEh rats were also used as normal control group. After 8 weeks of treatment, TGF-β1 expression was determined with immunohistochemical assay, and TGF-β1 concentration was evaluated with Microplate reader ABC-ELISA method. Resuits： Immunohistochemistry revealed expression of TGF-β1 was weak in normal control group, and obviously increased in model rats; however, the increased expression of TGF-β1 were down regulated in both high-dose and low dose groups, more obviously in low-dose group （P〈0.01） than that in high-dose group （P〈0.05）. Serum TGF-β1 concentration was significantly decreased in both high-dose and low dose group comparing with that in the model group （P〈0.01）. Conclusions： Both high and low dose of Softening and Resolving Hard Mass herbs in QF show effects on liver fibrosis and can regulate liver fibrosis cytokine TGFβ-1, this study confirmed the effects of Softening and Resolving Hard Mass herbs for hepatic fibrosis.