急性胰腺炎不同时期炎症因子的基因表达及意义

Changes of inflammatory cytokines during different stages of acute pancreatitis in rat model

目的:通过建立大鼠不同程度AP模型,观察AP病程中炎症因子的变化,为进一阐明AP发病机制提供实验依据。方法:选取健康雄性大鼠110只,随机分为假手术对照组(N)、急性水肿型胰腺炎组(AEP)和急性坏死型胰腺炎组(ANP),Realtime RT-PCR检测胰腺组织中IL-6、IL-10、TNF-α表达水平,ELISA法检测血清中IL-6、IL-10、TNF-α以及AMY含量检测。结果:模型组标本中IL-6和TNF-α均出现表达上调,其中TNF-α上调先于IL-6;IL-10呈先下调后又显著上调的趋势,血清中IL-6和TNF-α同样出现表达上调,IL-10呈先下调后又显著上调的趋势,ANP组血清中3种因子的水平变化幅度大于AEP组,24h后TNF-α表达水平显著高于12h水平,AEP及ANP组血清中AMY水平均呈逐渐升高,其中ANP组升高更为显著。血清中IL-6和TNF-α水平与AMY水平呈显著正相关。结论:炎症因子在AP进展中发挥了重要的作用,TNF-α是其中重要的环节,胰腺以外来源的炎症因子在AP激发的全身炎症反应中占重要地位。

Objective： To observe changes of inflammatory cytokines during different stages of acute pancreatitis （AP） in rat model. Methods： A total of 110 male rats were randomly divided into sham operation group （N）, acute edematous pancreatitis （AEP） and acute necrotizing pancreatitis （ANP）. Expresion level of IL-6, IL-10, TNF-α in pancreatic tissue was detected by realtime RT-PCR method, and their concentratinons were detected by ELISA assay. Results：Expression levels of IL-6 and TNF were up- regulated in samples of model group,and TNF-α was increased prior to IL-6. IL-10 was firstly reduced and then significant increased. Similar results were observed in serum. Changes in three factors in ANP group were greater than that in AEP group. TNF-α expression level after 24 h was significantly higher than that at 12 h. In AEP and ANP groups serum levels of AMY showed gradual increasing trend,which was more obvious in ANP group. Serum IL-6 and TNF-α levels and AMY levels were significantly correlated. Conclusion： Inflammatory factors play important roles in the progression of AP, especially TNF-α. Besides,inflammatory cytokines from non-pancreatic source play an important role in stimulating systemic inflammatory response in AP.