一种红树林链霉菌的胞外多糖免疫功能研究

Study on immunomodulatory effect of exopolysaccharide produced byStreptomyces from mangrove

目的:对一种分离自海南红树林链霉菌的胞外多糖(exopolysaccharide,EPS)进行了免疫调节活性研究。方法:以小鼠迟发型过敏反应、血清溶血素测定以及巨噬细胞吞噬实验探讨EPS的免疫调节活性,并以流式细胞仪测定脾脏淋巴细胞亚群。结果:EPS对小鼠免疫应答多项指标具有一定的正向调节作用(P﹤0.05);CD4+T细胞数量、CD4+/CD8+比率明显高于正常对照组。结论:此种产自链霉菌的EPS具有一定的免疫调节活性。

Objective. To explore the immunomodulatory effects of exopolysaccharide （EPS） from Streptomyces isolated from mangrove in Hainan province. Methods： The immunomodulatory effect was assayed by delayed type hypersensitivity reaction, the HC50 value and macrophage function assay in mice. Splenocyte subpopulaions were determined with flow cytometer. Results：Data showed that the EPS from Streptomyces could regulate immunity in mice （P 〈 0.05）. The percentage o f CD4＋ T cell and the ratio of CD4＋/CD8＋ in spleen of the EPS were higher than that in the normal control subjects （P 〈 0.05）. Conclusions： EPS from Streptomyces shows immunomodulatory effect.