胸水中腺癌细胞和反应性间皮细胞的形态结构定量判别分析

Quantitative discriminant analysis of morphological structure of adenocarcinoma cells and reactive mesothelial cells in pleural effusion

目的:定量揭示胸水中腺癌细胞(adenocarcinoma cell,ACC)的形态结构特征,筛选对ACC诊断分类有重要价值的尺寸和形状参数。方法:取胸水涂片中764个ACC(44例)和709个反应性间皮细胞(reactive mesothelial cell,RMC;39例),用计算机图像分析技术测试细胞和细胞核的尺寸和形状参数;其中尺寸参数包含面积(A)、长轴(dmaj)、短轴(dmin)、平均直径()、周长(C),形状参数包括规化形状因子(RFF)、形状不规则指数(FII)、核浆比(Rnp),对测试结果进行独立样本t检验和逐步判别分析。结果:与RMC相比,ACC的尺寸参数、FII、Rn p明显增大,而RFF明显减小(P<0.05)。逐步判别分析显示,dm a j,c、dm a j,n、An、Dn、Cn、FIIc进入判别函数,其符合率为75.9%。结论:形态结构参数dmaj,c、dmaj,n、An、n、Cn、FIIc对于胸水ACC的判别研究可能有价值。

Objective： To quantitatively anzlyze the morphological structure characteristics of ade- nocarcinoma cell （ACC） in pleural effusion, and to find dimension and form parameters which can be applied to identify ACC. Methods： A total of 764 ACC of 44 cases and 709 reactive mesothelial cells （RMC） of 39 cases were collected from pleural effusion smears. A technique of computer image analysis was applied to test the dimension and form parameters. Dimension parameters include Area（A）, Major axis （dmaj）, Minor axis（dmin）, Mean diameter（D）, Circumference（C） ;and form parameters include Regular form factor（RFF）, Form irregular index（FII）, Ratio of nucleus to cytoplasm（R,p）. Results were compared and put through independent-sample t test and stepwise discriminant analysis. Results： Compared with RMC, ACC showed significantly increased values of dimension parameters,FII, Rnp,while decreased RFF （P〈0.05）. Stepwise discriminant analysis showed dmaj、dmaj,n、An、Dn、Cn、FIIc entered discriminant function, and its coincidence rate was 75. 9%. Conclusions： Morphological structure parameters of dmaj、dmaj,n、An、Dn、Cn、FIIc may have important value in discriminant study on ACC of pleural effusion.