肠道高动力循环动物模型的建立

Establishment of animal model of intestinal hyperdynamic circulation

目的:建立肠道高动力循环动物模型,为研究门静脉高压(PHT)形成机制提供有利工具。方法:采用复合因素致肝纤维化方法,建立肠道高动力循环模型。选用雄性Wistar大鼠,分别在饲养第0、2、4、6、8周末取材,经肠系膜上静脉末端穿刺测压,动态观察门静脉压力(PVP)变化;肝脏HE、V.G染色及外周血丙氨酸氨基转移酶(ALT)、透明质酸(HA)测定来确定肝纤维化及硬化分期;测定外周血中的内皮素-1(ET-1)及肠管组织匀浆中一氧化氮(NO)的动态变化水平观察肠道高动力循环形成情况。并与正常对照组比较。结果:肠管组织匀浆中NO和外周血ET-1出现先降低后升高,到第8周末明显高于正常对照组;PVP的动态变化与NO、ET-1动态变化呈正性相关(r分别为0.900 7、0.851 4,P<0.05);NO/ET-1比值总体呈升高趋势。结论:通过复合因素致肝纤维化方法,可以建立大鼠肠道高动力循环模型。

Objective. To establish animal model of intestinal hyperdynamic circulation for research on mechanism of portal hypertension. Methods： Intestinal hyperdynamic circulation model was established by composite factors induced hepatic fibrosis. Male Wistar rats were selected, and were sampled at week 0, 2, 4, 6, 8. Dynamic portal venous pressure （PVP） was detected by puncturing end of superior mesenteric vein. Staging of hepatic fibrosis and sclerosis was determined by HE and VG staining of liver and measuring alanine aminotransferase （ALT） and hyaluronie acid （HA） in peripheral blood. Endothelin-1 （ET-1） in peripheral blood and NO in intestinal tissue homogenate were determined dynamically to observe hyperdynamic circulation. These results were compared with control group. Results. NO and ET-1 were decreased firstly and then increased,and were significantly higher than those of control group by the end of 8th week. Dynamic change of PVP was positively related with NO and ET-1 （r=0. 900 7,0. 851 4,P d0.05）. And NO/ET-1 was increased. Conclusions. Intestinal hyperdynamic circulation model can be established by composite factors induced liver fibrosis method.