催乳素、雌激素和胰岛素对奶牛乳腺上皮细胞中瘦素及其受体的影响

Influence of prolactin,estrogen and insulin,on leptin of mammary gland epithelial cell in dairy cow

以荷斯坦奶牛为实验材料,通过体外培养奶牛乳腺上皮细胞,添加催乳素、雌激素和胰岛素运用激光扫描共聚焦显微技术、Western Blot Analysis、Real-Time PCR等技术来观察乳腺上皮细胞中瘦素表达和泌乳功能的变化规律。结果表明,应用Western Blotting方法对泌乳期奶牛乳腺上皮细胞中瘦素和瘦素受体的蛋白表达量进行研究,催乳素、雌激素能显著提高瘦素及其受体的分泌,且催乳素高于雌激素,胰岛素影响不显著。荧光定量RT-PCR结果表明,催乳素和雌激素能提高瘦素及其受体mRNA表达量,胰岛素没有明显影响。

Mammary gland epithelial cells which have lactating function can be a cellular mode of research of mammary gland development and lactating regulation mechamsm. In this experiment, Holstein cows were used as materials, cow mammary gland epithelial cells were cultured in vitro and treated with estrogen, prolactin and insulin. Then leptin and its acceptor expressed by those cells and the change of lactation of those cells were observed and analyzed by laser scanning confocal microscopy, Western Blotting and Real-Time PCR.. The result of Westem Blotting analysis indicated, when cow mammary gland epithelial cells were treated with prolactin and estrogen, the expression of leptin and its acceptor by these cells were rose significant, but not changeed when treat insulin. The result of fluorescent quantitation Real-Time PCR indicated, the expression of mRNA of leptin and its acceptor in cow mammary gland epithelial cells were rose significant when these cells were treated with prolactin and estrogen, but no changes was occurred when these cells were treated with insulin.