摘要
以紫杉醇作为模型药物,旨在建立一种固相萃取-荧光分光光度法快速测定脂质体包封率的方法。分别采用水和75%甲醇对SampliQ C18固相萃取柱上的紫杉醇脂质体及其游离态进行洗脱分离,并在激发波长λex=244 nm、发射波长λem=311 nm条件下,利用荧光分光光度法测定游离紫杉醇的含量,从而间接计算紫杉醇脂质体的包封率。在本文建立的方法下,紫杉醇在0.006~0.078 mg/mL范围内线性关系良好(r=0.998),C18固相萃取柱对空白脂质体和游离紫杉醇的回收率均在97.5%以上。该方法测得自制紫杉醇脂质体包封率为50.8%(RAD=1.5%),与微柱离心法测得结果相比无显著性差异。实验结果表明,本方法可快速、简便地实现游离紫杉醇与其脂质体的分离测定,为脂质体的质量控制研究提供参考。
The objective of this study was to establish a method for the determination of the entrapment efficiency of liposomes by solid phase extraction-fiuorospectrophotometry. Paclitaxel was selected as the model drug. SampliQ C18 solid phase extraction columns were applied to separate free paclitaxel and its liposome using water and 75% methanol solution as elute solutions, respectively. A fluorospectrophotometries method ( λex=244 nm, λex=311 nm) was applied to determine the entrapment efficiency of paclitaxel liposome. Tile calibration curve was linear in the range of 0. 006-0. 078 mg/mL( r = 0. 998), and the recoveries of blank liposome and free pacli- taxel were all above 97.5%. The entrapment efficiency of paclitaxel liposome determined by this method was 50. 8% ( RAD = 1.5% ), which has no significant difference from those results measured by mini-column centrifu- gation. Solid phase extraction-fluorospectrophotometry could be applied to determine the entrapment efficiency of paclitaxel liposome rapidly and accurately and could be used to control the quality of paclitaxel liposome.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2012年第4期341-344,共4页
Journal of China Pharmaceutical University
基金
国家自然科学基金资助项目(No.30873197)~~
关键词
固相萃取
荧光分光光度法
紫杉醇脂质体
包封率
solid phase extraction
fluorospectrophotometry
paclitaxel liposome
entrapment efficiency