紫外-氯化锂复合诱变选育中性植酸酶高产菌株

Mutation Breeding of Neutral Phytase-Producing Strain Using Both UV Light and Lithium Chloride

以放射型根瘤杆菌为出发菌株,对其进行紫外-氯化锂(LiCl)复合诱变筛选,得到一株产中性植酸酶酶活力较高的菌株。当培养温度为30℃、pH值为7.0、接种量为10%时,经96h培养后,该菌株产中性植酸酶活力最高达到18.49U/mL,比原始菌株提高47.68%,且发酵周期缩短12h,该菌株具有良好的遗传稳定性。

Agrobacterium radiobacter AR01 was mutagenized using ultraviolet （UV） ircadiation and subsequent lithium chloride treatment to obtain a high-yield neutral phytase-producing strain. The neutral phytase activity of the strain reached up to 18.49 U/mL after 96 h cultivation at pH 7.0, 30 ℃ and an inoculum amount of 10%, which was 47.68% higher than that of the original strain. The fermentation cycle was shortened by 12 h. Moreover, the mutant strain showed good hereditary stability.