不同浓度沙门氏菌细胞生长分布规律研究

Modeling the Growth Distribution of Salmonella at Different Inoculum Levels

选用沙门氏菌SL1344在含20mg/kg甜菜碱和NaCl质量分数分别为0.0%、3.5%和5.0%的最低营养培养基(BMM)环境中分别对大量细胞、少量细胞进行研究,并对3.5%NaCl条件下单个细胞的生长进行研究。重点介绍进行少量细胞研究的一般理论和方法,以及单个细胞研究中单细胞生长流动槽成像系统的应用。结果发现,大量细胞生长分布具有决定性,单细胞生长分布具有随机性的特点,并通过不同的生长模型初步阐述其关系。

The basic minimal media （BMM） containing 0%, 3.5% or 5% NaCl and 20 mg/kg betaine Salmonella SL1344 were used to culture Salmonella enterica SL 1344 at an inoculum level of 103 CFU/mL in a constant temperature oven or at lower inoculum gradients in a Bioscreen for growth curve investigations. In addition, the growth of single cells under the condition of 3.5% NaCl was explored. The results showed that the growth distribution of cell colonies was determinative. By contrast, the growth distribution of single cells was stochastic. The relationship between the growth of single cells and cell colonies could be modeled.