白骨壤叶总黄酮的超声辅助提取及抗氧化活性研究

Ultrasonic-Assisted Extraction and Antioxidant Activity of Total Flavonoids from Avicennia marina Leaves

在单因素试验基础上,采用响应面分析法研究乙醇体积分数、固液比、超声功率和提取时间4个白骨壤叶总黄酮超声辅助提取因素,并比较白骨壤叶总黄酮的体外抗氧化作用。结果表明:白骨壤叶黄酮超声提取的最佳工艺条件为以粒度40～60目的白骨壤叶干粉为原料、乙醇体积分数64.39%、固液比1:28.20(g/mL)、提取功率293.63W、提取时间40.00min,该条件下白骨壤叶中总黄酮提取率为4.185%。白骨壤叶总黄酮体外清除羟自由基、超氧阴离子自由基和1,1-二苯基苦基苯肼(DPPH)自由基的IC50值分别0.232、0.829、23.692mg/L,其清除3种自由基的能力均高于VC。

On the basis of one-factor-at-a-time experiments, response surface analysis was employed to optimize four process parameters affecting the ultrasonic-assisted extraction of total flavonoids from Avicennia marina leaves including ethanol concentration, solid-to-solvent ratio, ultrasonic power and extraction time. Besides, the antioxidant activity in vitro of total flavonoids from Avicennia marina leaves was evaluated. The optimal extraction conditions were found as follows： dried Avicennia mMarina leaf powder （particle size： 40--60 mesh） was extracted with 64.39% aqueous ethanol solution at a solid-to-solvent ratio of 1：28.20 （g/mL） and a ultrasonic power of 293.63 W for 40.00 min. Under these conditions, the extraction yield of total flavonoids was 4.185%. The IC50 of the obtained extract against hydroxyl, superoxide anion and DPPH radicals were 0.232, 0.829 mg/L and 23.692 mg/L, respectively. All three types of radicals could be more effectively scavenged by the extract than VC.