固相萃取-高效液相色谱法测定果醋中7种有机酸

Determination of Seven Organic Acids in Fruit Vinegar by Solid-phase Extraction and High Performance Liquid Chromatography

目的:建立果醋中7种有机酸同时测定的高效液相色谱方法。方法:用水提取果醋中有机酸,后经LC-SAX固相萃取柱净化,以Atlantis T3 C18色谱柱进行分离,二极管阵列检测器检测。结果:在优化色谱条件下,7种有机酸在15min内分离良好,各组分回收率在97.5%～105.1%,相对标准偏差不大于1.31%,最低检出限在0.3 0～1.8 0μg/m L范围。结论:该方法具有简便、快速、准确等优点,可应用于果醋中有机酸的测定。

Objective： To develop an analytical method for the simultaneous determination of seven organic acids in fruit vinegar by high-performance liquid chromatography （HPLC）. Methods： Organic acids in fruit vinegar were extracted with water, cleaned up using an LC-SAX solid phase extraction cartridge, separated using an Atlantis T3 C18 column and detected using a diode- array detector. Results： Seven organic acids were separated within 15 minutes. The recovery rates of seven organic acids were in the range of 97.5 %--105.1% with RSD of less than 1.31%. The limits of detection were 0.30--1.80 μg/mL. Conclusion： This method provides a simple, rapid, accurate and suitable method for the simultaneous determination of organic acids in fruit vinegar.