HPLC法同时测定二妙丸中黄柏碱、木兰花碱、药根碱、盐酸巴马汀和盐酸小檗碱的含量

Simultaneous determination of phellodendrine,magnoflorine,jatrorrhizine,palmatine hydrochloride and berberine hydrochloride in Ermiao Pill by HPLC

目的建立二妙丸中黄柏碱、木兰花碱、药根碱、盐酸巴马汀和盐酸小檗碱的含量测定方法。方法采用高效液相色谱法同时测定二妙丸中黄柏碱、木兰花碱、药根碱、盐酸巴马汀和盐酸小檗碱的含量。色谱柱为phe-nomenex Luna C18柱(4.6 mm×250 mm,5μm),流动相A:乙腈,流动相B:0.02 mol/L磷酸二氢钾溶液-0.2%三乙胺溶液(磷酸调pH 3.0),梯度洗脱,流速为1.0 mL/min,柱温为30℃,检测波长为284 nm。结果在一定浓度范围内,黄柏碱、木兰花碱、药根碱、盐酸巴马汀和盐酸小檗碱峰面积与浓度呈良好的线性关系,平均加样回收率分别为98.09%,98.04%,99.75%,102.74%和104.84%,RSD分别为1.04%,1.36%,0.14%,1.83%和0.62%。结论该方法简便准确,专属性强,可作为二妙丸质量控制方法。

Purpose To establish a HPLC method for simultaneous determination of phellodendrine,magnoflorine,jatrorrhizine,palmatine hydrochloride and berberine hydrochloride in Ermiao Pill.Methods A phenomenex Luna C18 column was used,the mobile phase A was acetonitrile,the mobile phase B was the mixture of 0.02 mol/L potassium dihydrogen phosphate-0.2% triethylamine（pH 3.0）.The gradient elution was carried out with a flow rate of 1.0 mL/min.The column temperature was maintained at 30 ℃ and the detective wavelength was set at 284 nm.Results In a certain concentration range,the peak area of phellodendrine,magnoflorine,jatrorrhizine,palmatine hydrochloride and berberine hydrochloride showed a good linear relationship with the concentration.The average recoveries were 98.09%,98.04%,99.75%,102.74%,104.84% respectively and RSD were 1.04%,1.36%,0.14%,1.83%,0.62% respectively.Conclusion The method is simple,accurate and specific,can be used for the quality control of Ermiao Pill.