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5-氮-2'-脱氧胞苷对非霍奇金淋巴瘤p73基因甲基化的逆转作用 被引量：2

Reverse effect of 5-Aza-2′-deoxycytidine on p73 gene methylation in non-hodgkin′s lymphoma
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摘要 目的探讨5-氮-2'-脱氧胞苷对非霍奇金淋巴瘤(NHL)p73基因甲基化的逆转作用。方法用甲基化特异性PCR(MSP)方法对24例NHL p73基因启动子的甲基化进行检测,用5-氮-2'-脱氧胞苷处理部分NHL原代培养细胞后,检测p73 mRNA表达情况。结果 24例NHL中21例发生甲基化,甲基化阳性率高达87.5%;对照组(12例反应性增生淋巴结细胞及22例正常人外周血淋巴细胞)均未出现甲基化。部分未经治疗的NHL样本经5-氮-2'-脱氧胞苷处理后,p73 mRNA表达恢复。结论 p73基因启动子的甲基化可被5-氮-2'-脱氧胞苷逆转,导致因甲基化而被抑制的p73基因表达上调。
作者 裴晋红 柴文林 郭改娥 曹燕飞 汪军梅 栗学清
机构地区 长治医学院生物化学教研室 长治医学院附属和济医院
出处 《中国生化药物杂志》 CAS CSCD 北大核心 2012年第4期433-435,共3页 Chinese Journal of Biochemical Pharmaceutics
基金 国家自然科学基金资助项目(No.30770906)
关键词 5-氮-2'-脱氧胞苷 非霍奇金淋巴瘤 P73基因 甲基化
分类号 R961 [医药卫生—药理学]
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参考文献9

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同被引文献29

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中国生化药物杂志
2012年 第4期

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