光谱法研究顺铂与溶菌酶的相互作用

Spectroscopic Study on the Interaction of Cisplatin with Lysozyme

目的:研究顺铂与溶菌酶相互作用的光谱学特征。方法:应用光谱法研究顺铂与溶菌酶的相互作用机制;计算其结合常数、结合位点数和结合距离;根据热力学参数判断作用力类型;考察二者的相互作用对溶菌酶构象的影响。结果:顺铂对溶菌酶的荧光淬灭过程为生成复合物的静态淬灭;供能体与受能体之间的距离小于7nm,发生了非辐射能量转移;二者以氢键和范德华力结合为主;同步荧光光谱和三维荧光光谱表明结合反应影响了氨基酸残基所处的微环境。结论:顺铂能与溶菌酶结合并改变溶菌酶的构象。

OBJECTIVE：To study the spectroscopic characteristics of the interaction between cisplatin and lysozyme.METHODS：The interaction mechanism of cisplatin with lysozyme was investigated by spectroscopic method;the binding constants,binding sites and binding distance were calculated;the interaction force was estimated by thermodynamic parameters;the effects of their interactin on conformation change of lysozyme were investigated.RESULTS：The fluorescence quenching mechanism of cisplatin with lysozyme was due to the formation of cisplatin-lysozyme complex which resulted in static quenching procedure.The distance between donor and acceptor was less than 7 nm,which indicated that the energy nonradiative transfer occurred.Hydrogen bonds and van der Waals forces played a major role in the binding action.Synchronous and three-dimensional fluorescence spectra revealed that the interaction of cisplatin and lysozyme influenced the environments of amide acid residues.CONCLUSION：Cisplatin could bind with lysozyme and change the conformation of lysozyme.