安子合剂对ACA阳性先兆流产患者CD4^+CD25^+FOXP3^+Treg细胞免疫调节的影响

TheEffect of Anzi Mixture on Immune Regulation of CD4^+CD25^+FOXP3^+Treg Cells in ACA-positive Patients with Threatened Abortion

目的:观察安子合剂对ACA阳性先兆流产患者CD4+CD25+FOXP3+Treg细胞免疫调节的影响。方法:选取ACA阳性先兆流产患者为治疗组(27例),给服中药安子合剂,125ml,2次/日,连服4周;选取正常孕妇为对照组(15例)。检测患者治疗前后及正常孕妇外周血CD4+CD25+FOXP3+Treg细胞的比例,并测定患者治疗前后血清ACA指标、B超检查胚胎发育情况等。结果:治疗组患者治疗前CD4+CD25+FOXP3+Treg细胞的比例显著低于正常妊娠对照组(P<0.01);治疗后,其细胞比例显著高于治疗前(P<0.01)。27例患者中20例ACA阳性的转阴率达85.00%,7例ACA定量滴度较治疗前显著下降(P<0.01)。保胎的成功率达92.59%。结论:ACA阳性先兆流产患者外周血CD4+CD25+FOXP3+Treg细胞的比例低于正常妊娠妇女,存在免疫调节异常;安子合剂治疗ACA阳性先兆流产的作用机理之一与增加CD4+CD25+FOXP3+Treg细胞的比例、调节妊娠期机体免疫功能有关。

Objective： To observe the effect of Anzi Mixture on immune regulation of CD4^＋CD25^＋FOXP3^＋Treg cells in ACA-positive patients with threatened abortion. Methods ： Selecting ACA-positive patients with threatened abortion as treatment group （27cases）, 125ml Anzi Mixture were given, 2 times a day, consecutive administrated for 4 weeks; selecting normal pregnant women as the control group （15cases）. Detecting the percentage of peripheral blood CD4^＋CD25^＋FOXP3^＋Treg cells in the two groups, detecting the indicators of ACA before and after treatment by Anzi Mixture, B-ultrasound embryo development and so on. Results： Before treatment, the proportion of CD4^＋CD25^＋FOXP3^＋Treg cells in ACA-pesitive patients with threatened abortion was significantly lower than the control group （P〈0.01） ; after treatment of Anzi Mixture, the proportion of CD4^＋CD25^＋FOXP3^＋Treg cells was significantly higher than before treatment （ P〈0. 01 ）. The ACA negative rate of the qualitative indicators was 85.00% , 7 cases of quantitative indicators of ACA titers were significantly decreased compared with before treatment （P 〈 0.01）. Success rate of miscarriage was 92. 59%. Conclusion： CD4^＋CD25^＋FOXP3^＋Treg cells percentage were lower than nor-mal pregnant women in peripheral blood of ACA-positive patients with threatened abortion who exist abnormal immune regulation. One of the mechanisms of Anzi Mixture is to increase the percentage of CD4^＋CD25^＋FOXP3^＋Treg cells and to regulate the immune function of pregnancy.