水分胁迫条件下钙赤合剂和磷浸种对冬小麦幼苗净光合速率和抗氧化系统的影响

Effects of Seed Soaking with Ca-GA Mixture and Phosphorus on Photosynthetic Rate and Antioxidant System of Wheat Seedlings under Water Stress Condition

以豫麦49为研究材料,在水分胁迫条件下研究钙赤合剂(Ca-GA)和磷(P)浸种对幼苗净光合速率(Pn)、水分利用效率(WUE)、渗透调节物质脯氨酸(Pro)、膜脂过氧化物丙二醛(MDA)及抗氧化酶系统的影响。结果表明:与清水浸种处理相比,Ca-GA浸种和P浸种处理后的幼苗在重度水分胁迫条件下其光合速率和水分利用效率增加,MDA含量降低,Pro含量增加,过氧化物酶(POD)、超氧化物歧化酶(SOD)及过氧化氢酶(CAT)活性增强,抗坏血酸-过氧化物酶(APX)活性变化不大,抗坏血酸(AsA)和还原型谷胱甘肽(GSH)含量升高。以上结果表明:Ca-GA浸种和P浸种处理均有效降低了水分胁迫导致的抗氧化系统紊乱,使幼苗保持一定的光合速率和较高的水分利用效率。总体上看,Ca-GA浸种和P浸种可有效提高小麦幼苗抗旱性。

In order to investigate the effect of water stress on plant photosynthesis and anti-oxidant system, the net photosynthetic rate, water use efficiency （ WUE）, malondialdehyde （MDA） and proline （Pro） concent, and anti-oxi- dant enzyme activities in leaves of wheat seedling treated with seed soaking with Ca-GA and/or P were studied un- der water stress conditions. The results indicated that seed soaking with Ca-GA or P decreased MDA content and increased （SOD） （APX） treated Pro, ascorbic acid （ AsA）, reduced glutathione （GSH） concent and peroxidase （ POD ）, superoxide dismutase and hydrogen peroxidase （CAT） activity, while had no of seedlings under severe water stress （SWS） condition. obvious effect on activity of ascorbate peroxidase Compared with the control treatment, the seedlings with seed soaking with Ca-GA or P maintained higher net photosynthetic rates and WUE under SWS condi- tion. In conclusion, seed soaking der water stress conditions. with Ca-GA or P could enhance anti-drought characteristics of wheat seedlings under water stress conditions.