外源物质对小麦芽期抗旱性的影响

Effects of Exogenous Substances on the Drought Resistance in Wheat During Germinating Stage

为筛选有利于提高渗透胁迫下小麦抗旱性的化学物质和最佳组合,以豫麦49为材料,在实验室中用15%PEG-6000模拟干旱,分别用不同浓度的磷酸二氢钾、赤霉素、葡萄糖、矮壮素及其混合液处理小麦种子。测定了各处理的芽长、根长、根数等形态指标和可溶性糖、丙二醛、脯氨酸含量等生理指标。结果表明,在PEG胁迫下,外源物质能不同程度地增加小麦幼苗的芽长、根长、根数等形态指标以及可溶性糖和脯氨酸含量,降低MDA的累积量,不同外源物质对水分亏缺的调节能力存在差异,其中外源物质的混合处理比单独处理的小麦表现出更强的抗旱能力。用0.2%KH2PO4、25mg/kg GA和500mg/kg葡萄糖的混合液处理小麦种子能增强小麦芽期的抗旱性。

In order to screening out the chemical substances and the optimal treatment combination for drought resistance of wheat under osmotic stress,Yumai 49 was used as experimental material in the laboratory.Effects of seed treatment with phosphate dihydrogen potassium,gibberellin,glucose,chlorocholine chlorid(CCC) and their mixture,respectively on germination length,root length,root number and soluble sugar,malondialdehyde(MDA),proline content were studied under 15% PEG simulating drought condition during germinating stage.The results indicated that under PEG stress,the exogenous substances could increase the length of wheat buds and roots,the amount of roots and the content of soluble sugar and proline,and reduced the content of MDA.Different exogenous substances varied in the abilities of adjusting to water deficiency.In which,the seeds treated by intermixture of exogenous substances showed stronger germination capacity.The wheat seeds treated with the mixture of 0.2% KH2PO4,25mg/kg GA and 500mg/kg glucose could enhance drought resistance of wheat during germinating stage.