摘要
近年来,在对肺炎克氏杆菌(Klebsiella pneumoniae)nifA产物研究的基础上已建立了固氮基因调控的模型。固氮基因(nif)的表达受氮调节系统ntr和nifAL操纵子的两个系统的调控。本实验将固氮基因nifHDK启动子、aphA-6基因和rbcL3'构建aphA-6基因表达盒,克隆到pSK.KmR载体,将其转入到大肠杆菌中验证nifHDK启动子的表达活性。结果表明,只有在转入外源nifA基因时,nifHDK启动子才具有转录活性,aphA-6基因才能表达。验证了NifA蛋白和σ54因子是固氮基因的正向调节蛋白,为下一步固氮生物工程研究提供了理论基础。
We have made the model of nitrogen fixation ( nif ) gene regulation on the basis of Klebsiella pneu-moniae nifA protein. The expression of nil gene were regulated by ntr and nifAL. The plasmid pSKKmR was construct by insertion of pnifHDK, aphA-6 and rbcL3', then it was transfered into E. coli for testing the expres-sion of pnifHDK. The result illustrated that pnifHDK cant promote gene expression and AphA-6 gene cant ex-press without NifA protein. The result proved that NifA protein and δ54 factor were the up-regulation protein of nif gene. The result also made theoretics foundation of biological nitrogen fixation.
出处
《辽宁农业科学》
2012年第1期29-33,共5页
Liaoning Agricultural Sciences
关键词
生物固氮
NIFA基因
固氮基因
Biological nitrogen fixation
NifA Gene
Nitrogen Fixation Gene