高效液相色谱-化学发光法测定人血清及动物组织中的己烯雌酚

Determination of diethylstilbestrol in human serum and animal tissues by HPLC separation with chemiluminescence detection

基于己烯雌酚在酸性介质中对Ce(Ⅳ)和Rh6G的化学发光体系的增敏作用,建立了人血清及动物组织中己烯雌酚的高效液相色谱/化学发光检测方法。色谱柱为Welchrom-C18柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(75:25,V/V,pH6.0)。己烯雌酚在1.0×10-8～2.5×10-6g/mL范围内呈良好线性,在优化的实验条件下最低检出限为3.0×10-9g/mL。对5.0×10-7g/mL的己烯雌酚进行11次平行测定,其相对标准偏差为2.1%。

Based on the enhanced sensitivity of the cerium（IV）-rhodamine 6G chemiluminescence system by diethylstilbestrol,a method for the determination of diethylstilbestrol in human serum and animal tissues by HPLC separation coupled with chemiluminescence（CL） detection was described.The separation was carried out on a Welchrom-C18 column（4.6 mm×250 mm,5 μm） with mobile phase consisting of methanol-H2O（75：25,V/V,pH6.0）.Under the optimum conditions,the CL intensity has a linear relationship with the concentration of naproxen in the range of 1.0×10-8～2.5×10-6 g/mL with the detection limit of 3.0×10-9 g/mL（3σ）.The relative standard deviation for 5.0×10-7 g/mL diethylstilbestrol was 2.1%（n=11）.