摘要
利用ISSR分子标记分析茄子种质多样性,从100条引物中筛选出20条能够扩增出多态性条带稳定清晰的ISSR引物,对48份茄子材料的DNA进行扩增,共获得了186条谱带,其中多态性带153条(占所有谱带的82.2%).利用UPGMA法建立了48份茄子及其近缘野生种间的遗传关系聚类图.聚类结果表明,48份供试种质间的遗传相似系数分布在0.52～0.98之间,可将其分为5大类群.其中又将44份栽培品种分为5组,其品种间相似遗传系数在0.84～0.98之间,表明栽培品种的遗传背景比较窄.聚类结果与茄子的果形以及地理来源有一定的相关性,而与果色呈现不完全相关.
The genetic relationship and diversity of 44 eggplants, Solanum melongena L., germplasms and 4 wild relatives were.analyzed by ISSR method . One hundred and fifty-three of 186 bands were amplified by 20 ISSR primers, about 82.2% being polymorphisms. Fourty-eight germplasms were clustered into 5 groups by genetic similar index from 0. 52 to 0. 98, and the wild species were different from eggplants. Also 44 eggplant cuhivars had a high genetic similarity and a narrow genetic diversity. At the same time, the results showed that the cluster was related to the fruit shape of eggplants and origin areas but not to the fruit colour.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2012年第3期296-300,共5页
Journal of South China Agricultural University
基金
广东省自然科学基金(9151064201000063)
国家自然科学基金(30972005)
广东省自然科学基金研究团队项目(S2011030001410)
关键词
茄子
ISSR
遗传多样性
相关分析
eggplant
ISSR
genetic diversity
correlation analysis
