代谢综合征大鼠下丘脑室旁核神经元型一氧化氮合酶的表达

Expression of the neuronal nitric oxide synthase in the paraventricular hypothalamic nucleus in rats with metabolic syndrome

目的:观察代谢综合征大鼠下丘脑室旁核(para-ventricular hypothalamic nucleus,PVN)内神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)表达的变化,探讨PVN内一氧化氮(nitric oxide,NO)在代谢综合征发病中的作用机制。方法:高脂高糖诱导大鼠代谢综合征24周,应用免疫组织化学染色方法观察代谢综合征大鼠PVN内nNOS的表达。结果:与对照组相比较,模型组PVN内nNOS表达明显增加(P<0.01)。结论:PVN内nNOS的表达在代谢综合征大鼠中明显升高,nNOS活性改变可能与代谢综合征神经内分泌改变有关。

Objective：To examine the changes of neuronal nitric oxide synthase（nNOS） expression in paraventricular hypothalamic nucleus（PVN） in rats with metabolic syndrome,and explore the mechanisms of nitric oxide（NO） in PVN in inducing the metabolic syndrome.Methods：A high-fat,refined-carbohydrate diet was given to the rats for 24 weeks to induce metabolic syndrome.Immunohistostaining was performed to examine the expression of nNOS in PVN.Results：Compared with the normal control group,the expression of nNOS in the model group were up-regulated significantly（P〈0.01）.Conclusion：The fact that evidently up-regulated nNOS expression in PVN was seen in rats with metabolic syndrome suggest that the changes of nNOS activities may be involved in variation of the cerebral neuroendocrine.