超高效液相色谱同时测定食品中4种工业染料

Simultaneous determination of four industrial dyes in foods by ultra high performance liquid chromatography

目的建立同时测定食品中酸性橙、碱性橙、碱性嫩黄、罗丹明B工业染料的方法。方法样品用水-5%氨水乙腈-正己烷混合溶剂提取净化,采用ZORBAX Extend C18(2.1 mm i.d.×50 mm,1.8μm)分离,以乙腈—乙酸铵缓冲液梯度洗脱,流速为0.5 ml/min,二极管阵列检测器检测酸性橙(λ=485 nm)、碱性橙及碱性嫩黄(λ=435nm)、罗丹明B(λ=548 nm)。结果 4种组分在0.1～10.0 mg/L范围内有良好的线性关系(r>0.999),检测限为0.006 4～0.019 mg/kg。在番茄沙司、腊肠、辣椒油3种不同食品基质中平均加标回收率为70.0%～102.7%,相对标准偏差为0.5%～3.1%。结论方法快速,简单,可应用于食品中酸性橙、碱性橙、碱性嫩黄、罗丹明B 4种工业染料的同时检测。

Objective To establish a method for the simultaneous determination of Orange 2, Aurnine O, Chrysoidine G and Rhodamine B in foods by Uhra High Performance Liquid Chromatography （ UHPLC ）. Methods Samples were extracted and cleaned up with water -5% ammonia acetonitrile-n-Hexane mixture solvents. UHPLC separation was achieved by using ZORBAX Extend Cls （2. 1 mmi. d. x 50 mm, 1.8 μm） with an mobile phase consisting of acetonitrile and acetate buffer, and gradient program was used at a flow rate of 0. 5 ml/min. The detection wavelength was set at 485nm for Orange 2, 435 nm for aurnine O and chrysoidine G, while 548 nm for rhodamine B. Results There was a good linear relationship （ r 〉 0. 999） in the range of 0. 1-10 mg/L for these four dyes. The limits of detection （LOD） were from O. 006 4 to 0. 019 mg/kg respectively. The average recoveries for all four dyes in tomato sauce, sausage and chili powderwere ranged 70. 0% -102. 7% , the relative standard deviations （RSDs） were 0. 5% -3.1%. Conclusion The method is rapid, simple and could be applied for the simultaneous determination of Orange 2, Aurnine O, Chrysoidine G and Rhodamine B in different kinds of foods.